Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5059072..5059569 Replicon chromosome
Accession NZ_CP032801
Organism Escherichia coli strain ERL06-2442

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D8Z71_RS25885 Protein ID WP_001173343.1
Coordinates 5059072..5059245 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag D8Z71_RS25890 Protein ID WP_000398619.1
Coordinates 5059273..5059569 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z71_RS25875 5055742..5057199 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
D8Z71_RS25880 5057436..5058953 + 1518 WP_001295074.1 lysine--tRNA ligase -
D8Z71_RS25885 5059072..5059245 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D8Z71_RS25890 5059273..5059569 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D8Z71_RS25895 5059796..5060068 - 273 WP_000405647.1 N-acetyltransferase -
D8Z71_RS25900 5060080..5060310 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
D8Z71_RS25905 5060491..5062122 + 1632 WP_001216477.1 sensor histidine kinase -
D8Z71_RS25910 5062119..5062838 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T112430 WP_001173343.1 NZ_CP032801:c5059245-5059072 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T112430 NZ_CP032801:c5059245-5059072 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT112430 WP_000398619.1 NZ_CP032801:c5059569-5059273 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT112430 NZ_CP032801:c5059569-5059273 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References