Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2618661..2619132 Replicon chromosome
Accession NZ_CP032801
Organism Escherichia coli strain ERL06-2442

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag D8Z71_RS13500 Protein ID WP_001303511.1
Coordinates 2618661..2618939 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag D8Z71_RS13505 Protein ID WP_001302048.1
Coordinates 2618941..2619132 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z71_RS13470 2615597..2616682 - 1086 Protein_2572 exonuclease -
D8Z71_RS13475 2616775..2616966 - 192 WP_001090200.1 DUF1482 family protein -
D8Z71_RS13480 2616963..2617151 - 189 WP_000449192.1 cell division inhibition protein DicB -
D8Z71_RS13490 2617720..2617938 - 219 WP_001171930.1 hypothetical protein -
D8Z71_RS13495 2618010..2618309 - 300 WP_001240334.1 hypothetical protein -
D8Z71_RS13500 2618661..2618939 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z71_RS13505 2618941..2619132 - 192 WP_001302048.1 hypothetical protein Antitoxin
D8Z71_RS13510 2619153..2619524 - 372 WP_001169686.1 hypothetical protein -
D8Z71_RS13515 2619622..2619924 + 303 WP_000172738.1 transcriptional regulator -
D8Z71_RS13520 2619921..2620346 + 426 WP_000693943.1 Rha family transcriptional regulator -
D8Z71_RS13525 2620369..2621331 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
D8Z71_RS13530 2621338..2622078 + 741 WP_000788938.1 ATP-binding protein -
D8Z71_RS13535 2622104..2622873 + 770 Protein_2584 DUF1627 domain-containing protein -
D8Z71_RS13540 2622889..2623284 + 396 WP_001118159.1 DUF977 family protein -
D8Z71_RS13545 2623341..2623925 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleA/espI 2602469..2660818 58349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T112418 WP_001303511.1 NZ_CP032801:c2618939-2618661 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T112418 NZ_CP032801:c2618939-2618661 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT112418 WP_001302048.1 NZ_CP032801:c2619132-2618941 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT112418 NZ_CP032801:c2619132-2618941 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References