Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 2323..2870 Replicon plasmid pERL06-2497-2
Accession NZ_CP032799
Organism Escherichia coli strain ERL06-2497

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag D8Z72_RS28640 Protein ID WP_000969996.1
Coordinates 2589..2870 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag D8Z72_RS28635 Protein ID WP_000079928.1
Coordinates 2323..2592 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z72_RS28610 1..261 + 261 WP_000083819.1 replication regulatory protein RepA -
D8Z72_RS28620 486..560 + 75 WP_001365705.1 RepA leader peptide Tap -
D8Z72_RS28625 553..1410 + 858 WP_001554370.1 incFII family plasmid replication initiator RepA -
D8Z72_RS29280 2110..2268 + 159 WP_192795837.1 hypothetical protein -
D8Z72_RS28635 2323..2592 + 270 WP_000079928.1 hypothetical protein Antitoxin
D8Z72_RS28640 2589..2870 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8Z72_RS28645 2955..3302 + 348 WP_000142452.1 hypothetical protein -
D8Z72_RS28650 3320..3910 - 591 WP_042004720.1 hypothetical protein -
D8Z72_RS28655 3907..4167 - 261 WP_019842588.1 hypothetical protein -
D8Z72_RS28660 4736..5149 + 414 WP_000465034.1 hypothetical protein -
D8Z72_RS28665 5151..5933 + 783 WP_001164199.1 site-specific integrase -
D8Z72_RS28670 6106..6459 + 354 WP_001401712.1 colicin M immunity protein -
D8Z72_RS28675 6509..7324 - 816 WP_000449473.1 lipid II-degrading bacteriocin colicin M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..63089 63089


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T112392 WP_000969996.1 NZ_CP032799:2589-2870 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T112392 NZ_CP032799:2589-2870 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTAGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT112392 WP_000079928.1 NZ_CP032799:2323-2592 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT112392 NZ_CP032799:2323-2592 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References