Detailed information of TA system TA112346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2646646..2647117
• Replicon: chromosome
• Accession: NZ_CP032795
• Organism: Escherichia coli strain ERL06-2503

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: D8Z73_RS13680
• Protein ID: WP_001303511.1
• Coordinates: 2646646..2646924 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: D8Z73_RS13685
• Protein ID: WP_001302048.1
• Coordinates: 2646926..2647117 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z73_RS13640	2641653..2641837	-	185	Protein_2607	DUF3596 domain-containing protein	-
D8Z73_RS13645	2641872..2642123	-	252	WP_000005552.1	excisionase family protein	-
D8Z73_RS13650	2642196..2644667	-	2472	WP_000048458.1	exonuclease	-
D8Z73_RS13655	2644760..2644951	-	192	WP_001090200.1	DUF1482 family protein	-
D8Z73_RS13660	2644948..2645136	-	189	WP_000449192.1	cell division inhibition protein DicB	-
D8Z73_RS13670	2645705..2645923	-	219	WP_001171930.1	hypothetical protein	-
D8Z73_RS13675	2645995..2646294	-	300	WP_001240334.1	hypothetical protein	-
D8Z73_RS13680	2646646..2646924	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8Z73_RS13685	2646926..2647117	-	192	WP_001302048.1	hypothetical protein	Antitoxin
D8Z73_RS13690	2647138..2647509	-	372	WP_001169686.1	hypothetical protein	-
D8Z73_RS13695	2647607..2647909	+	303	WP_000172738.1	transcriptional regulator	-
D8Z73_RS13700	2647906..2648331	+	426	WP_000693943.1	Rha family transcriptional regulator	-
D8Z73_RS13705	2648354..2649316	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
D8Z73_RS13710	2649323..2650063	+	741	WP_000788938.1	ATP-binding protein	-
D8Z73_RS13715	2650089..2650858	+	770	Protein_2621	DUF1627 domain-containing protein	-
D8Z73_RS13720	2650874..2651269	+	396	WP_001118159.1	DUF977 family protein	-
D8Z73_RS13725	2651326..2651910	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2638360..2688803	50443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T112346 WP_001303511.1 NZ_CP032795:c2646924-2646646 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T112346 NZ_CP032795:c2646924-2646646 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT112346 WP_001302048.1 NZ_CP032795:c2647117-2646926 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT112346 NZ_CP032795:c2647117-2646926 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0