Detailed information of TA system TA112321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4809779..4810374
• Replicon: chromosome
• Accession: NZ_CP032793
• Organism: Escherichia coli strain NZRM3614

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: D8Z74_RS24335
• Protein ID: WP_000239579.1
• Coordinates: 4809779..4810129 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: D8Z74_RS24340
• Protein ID: WP_001223210.1
• Coordinates: 4810123..4810374 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z74_RS24315	4805225..4806247	-	1023	WP_001301928.1	ABC transporter permease	-
D8Z74_RS24320	4806261..4807763	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
D8Z74_RS24325	4807903..4808859	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
D8Z74_RS24330	4809169..4809699	+	531	WP_001691111.1	inorganic diphosphatase	-
D8Z74_RS24335	4809779..4810129	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
D8Z74_RS24340	4810123..4810374	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
D8Z74_RS24345	4810586..4810927	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
D8Z74_RS24350	4810930..4814709	-	3780	WP_001691110.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T112321 WP_000239579.1 NZ_CP032793:c4810129-4809779 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T112321 NZ_CP032793:c4810129-4809779 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT112321 WP_001223210.1 NZ_CP032793:c4810374-4810123 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT112321 NZ_CP032793:c4810374-4810123 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8