Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48368..48893 Replicon plasmid pNZRM4165
Accession NZ_CP032792
Organism Escherichia coli strain NZRM4165

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag D8Z75_RS27600 Protein ID WP_001159868.1
Coordinates 48588..48893 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag D8Z75_RS27595 Protein ID WP_000813634.1
Coordinates 48368..48586 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z75_RS27560 43651..43881 + 231 WP_010891288.1 hypothetical protein -
D8Z75_RS27565 44002..44742 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
D8Z75_RS27570 45027..46004 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
D8Z75_RS27580 46412..46612 - 201 WP_000708307.1 hypothetical protein -
D8Z75_RS27585 46609..47229 - 621 WP_001248529.1 hypothetical protein -
D8Z75_RS27590 47226..47909 - 684 WP_010891291.1 DNA-binding protein -
D8Z75_RS27595 48368..48586 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
D8Z75_RS27600 48588..48893 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
D8Z75_RS27605 48894..49700 + 807 WP_000016989.1 site-specific integrase -
D8Z75_RS27610 50419..51174 + 756 WP_000852148.1 replication initiation protein RepE -
D8Z75_RS27615 51762..52928 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..90310 90310


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T112287 WP_001159868.1 NZ_CP032792:48588-48893 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T112287 NZ_CP032792:48588-48893 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT112287 WP_000813634.1 NZ_CP032792:48368-48586 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT112287 NZ_CP032792:48368-48586 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References