Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4111860..4112478 Replicon chromosome
Accession NZ_CP032791
Organism Escherichia coli strain NZRM4165

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D8Z75_RS21260 Protein ID WP_001291435.1
Coordinates 4112260..4112478 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D8Z75_RS21255 Protein ID WP_000344800.1
Coordinates 4111860..4112234 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z75_RS21245 4106949..4108142 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D8Z75_RS21250 4108165..4111314 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
D8Z75_RS21255 4111860..4112234 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D8Z75_RS21260 4112260..4112478 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
D8Z75_RS21265 4112650..4113201 + 552 WP_000102553.1 maltose O-acetyltransferase -
D8Z75_RS21270 4113317..4113787 + 471 WP_000136192.1 YlaC family protein -
D8Z75_RS21275 4113951..4115501 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D8Z75_RS21280 4115543..4115896 - 354 WP_000878140.1 DUF1428 domain-containing protein -
D8Z75_RS21290 4116275..4116586 + 312 WP_000409911.1 MGMT family protein -
D8Z75_RS21295 4116619..4117191 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T112278 WP_001291435.1 NZ_CP032791:4112260-4112478 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T112278 NZ_CP032791:4112260-4112478 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT112278 WP_000344800.1 NZ_CP032791:4111860-4112234 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT112278 NZ_CP032791:4111860-4112234 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References