Detailed information of TA system TA112261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1955260..1955898
• Replicon: chromosome
• Accession: NZ_CP032791
• Organism: Escherichia coli strain NZRM4165

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D8Z75_RS09625
• Protein ID: WP_000813794.1
• Coordinates: 1955722..1955898 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D8Z75_RS09620
• Protein ID: WP_001270286.1
• Coordinates: 1955260..1955676 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z75_RS09600	1950412..1951353	-	942	WP_001251315.1	ABC transporter permease	-
D8Z75_RS09605	1951354..1952367	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
D8Z75_RS09610	1952385..1953530	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
D8Z75_RS09615	1953775..1955181	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
D8Z75_RS09620	1955260..1955676	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D8Z75_RS09625	1955722..1955898	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D8Z75_RS09630	1956120..1956350	+	231	WP_000494244.1	YncJ family protein	-
D8Z75_RS09635	1956442..1958403	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D8Z75_RS09640	1958476..1959012	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
D8Z75_RS09645	1959065..1960276	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T112261 WP_000813794.1 NZ_CP032791:c1955898-1955722 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T112261 NZ_CP032791:c1955898-1955722 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT112261 WP_001270286.1 NZ_CP032791:c1955676-1955260 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT112261 NZ_CP032791:c1955676-1955260 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB