Detailed information of TA system TA112248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4916309..4916904
• Replicon: chromosome
• Accession: NZ_CP032789
• Organism: Escherichia coli strain NZRM4169

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: D8Z76_RS25160
• Protein ID: WP_000239579.1
• Coordinates: 4916309..4916659 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: D8Z76_RS25165
• Protein ID: WP_001223210.1
• Coordinates: 4916653..4916904 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z76_RS25140	4911761..4912777	-	1017	WP_151539055.1	ABC transporter permease	-
D8Z76_RS25145	4912791..4914293	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
D8Z76_RS25150	4914433..4915389	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
D8Z76_RS25155	4915699..4916229	+	531	WP_000055075.1	inorganic diphosphatase	-
D8Z76_RS25160	4916309..4916659	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
D8Z76_RS25165	4916653..4916904	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
D8Z76_RS25170	4917116..4917457	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
D8Z76_RS25175	4917460..4921239	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T112248 WP_000239579.1 NZ_CP032789:c4916659-4916309 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T112248 NZ_CP032789:c4916659-4916309 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT112248 WP_001223210.1 NZ_CP032789:c4916904-4916653 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT112248 NZ_CP032789:c4916904-4916653 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8