Detailed information of TA system TA112202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 3264600..3265162
• Replicon: chromosome
• Accession: NZ_CP032757
• Organism: Lactiplantibacillus pentosus strain DSM 20314

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F6IUY4
• Locus tag: LP314_RS15240
• Protein ID: WP_050339912.1
• Coordinates: 3264815..3265162 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LP314_RS15235
• Protein ID: WP_050339935.1
• Coordinates: 3264600..3264815 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LP314_RS15220	3260212..3262254	+	2043	WP_056952649.1	KUP/HAK/KT family potassium transporter	-
LP314_RS15225	3262322..3262963	-	642	WP_050339934.1	N-acetyltransferase	-
LP314_RS15230	3263108..3263839	-	732	WP_050339911.1	hypothetical protein	-
LP314_RS15235	3264600..3264815	+	216	WP_050339935.1	hypothetical protein	Antitoxin
LP314_RS15240	3264815..3265162	+	348	WP_050339912.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LP314_RS15245	3265582..3266625	-	1044	WP_021337327.1	AI-2E family transporter	-
LP314_RS15250	3267078..3268202	+	1125	WP_050339913.1	vitamin B12 independent methionine synthase	-
LP314_RS15255	3268357..3268740	-	384	WP_050339914.1	multidrug efflux SMR transporter	-
LP314_RS15260	3268757..3269065	-	309	WP_003639898.1	quaternary ammonium transporter	-
LP314_RS15265	3269441..3270079	-	639	WP_050339915.1	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-93)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13406.29 Da
• Isoelectric Point: 9.4892

>T112202 WP_050339912.1 NZ_CP032757:3264815-3265162 [Lactiplantibacillus pentosus]
MTYLPKQKDIIWIDFDPQRGREIKKRRPAVILSSNLYTQNTGFVIVSPITSTIHDLPGYFSLNGYDIHGQVVAAQIYSFD
ARPKAGRNITYIETMRNVDFYRVAQTVYYNFDFPF

Nucleotide

• Length: 348 bp

>T112202 NZ_CP032757:3264815-3265162 [Lactiplantibacillus pentosus]
ATGACTTATTTGCCTAAGCAAAAAGATATCATCTGGATTGATTTTGATCCGCAACGCGGCCGCGAAATAAAAAAGCGCCG
TCCTGCTGTAATCCTTAGTAGTAACCTCTACACCCAGAACACCGGCTTTGTAATTGTTTCACCAATTACGTCTACCATTC
ATGATTTGCCCGGCTACTTTAGTCTCAATGGCTACGATATCCATGGACAAGTTGTCGCTGCACAGATTTATTCGTTTGAC
GCGAGGCCAAAAGCGGGGCGAAATATCACATATATCGAAACCATGCGCAACGTAGATTTTTATCGCGTCGCGCAGACAGT
GTACTACAACTTTGACTTTCCATTCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8044.14 Da
• Isoelectric Point: 9.6576

>AT112202 WP_050339935.1 NZ_CP032757:3264600-3264815 [Lactiplantibacillus pentosus]
IEVKTVSRGNSLALSLPKDGRFKKGQRWLLIPSKDGESYTLVPRIENPYAGPKSKQPMTEAWSDVNWNEVE

Nucleotide

• Length: 216 bp

>AT112202 NZ_CP032757:3264600-3264815 [Lactiplantibacillus pentosus]
ATTGAAGTAAAAACAGTTAGCCGTGGCAACTCATTGGCATTAAGTCTGCCTAAAGACGGCCGTTTTAAAAAAGGCCAACG
CTGGTTACTCATCCCATCAAAAGATGGGGAGTCATATACACTCGTACCACGTATTGAAAATCCTTATGCTGGTCCCAAGT
CAAAACAGCCAATGACTGAAGCGTGGTCTGATGTTAACTGGAATGAGGTCGAATAA

Structures

Toxin

• AlphaFold DB: F6IUY4