Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 32389..33526 Replicon plasmid pEC369
Accession NZ_CP032740
Organism Enterococcus casseliflavus strain EC-369

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag D8N35_RS17790 Protein ID WP_002343781.1
Coordinates 32389..33252 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag D8N35_RS17795 Protein ID WP_000301765.1
Coordinates 33254..33526 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8N35_RS17765 27676..28188 + 513 Protein_28 IS30 family transposase -
D8N35_RS17770 28260..29873 - 1614 WP_002333004.1 hypothetical protein -
D8N35_RS17775 29897..30778 - 882 WP_002326774.1 ABC transporter ATP-binding protein -
D8N35_RS17780 31089..31685 + 597 WP_002326773.1 TetR/AcrR family transcriptional regulator -
D8N35_RS17785 31770..32267 - 498 Protein_32 DnaJ domain-containing protein -
D8N35_RS17790 32389..33252 - 864 WP_002343781.1 zeta toxin family protein Toxin
D8N35_RS17795 33254..33526 - 273 WP_000301765.1 antitoxin Antitoxin
D8N35_RS17800 33543..33752 - 210 WP_000527317.1 peptide-binding protein -
D8N35_RS17805 33857..34753 - 897 WP_002343780.1 ParA family protein -
D8N35_RS17810 34981..36609 - 1629 WP_002343779.1 IS1182 family transposase -
D8N35_RS17815 36713..37351 - 639 WP_002343778.1 hypothetical protein -
D8N35_RS17820 37404..37694 - 291 WP_002343777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(6)-Ia / erm(B) / aph(3')-III - 1..91960 91960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32412.95 Da        Isoelectric Point: 6.6610

>T112200 WP_002343781.1 NZ_CP032740:c33252-32389 [Enterococcus casseliflavus]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIIIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T112200 NZ_CP032740:c33252-32389 [Enterococcus casseliflavus]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTATCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTACGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT112200 WP_000301765.1 NZ_CP032740:c33526-33254 [Enterococcus casseliflavus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT112200 NZ_CP032740:c33526-33254 [Enterococcus casseliflavus]
ATGGCAGTTACCTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTCAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References