112199

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3267159..3267793 Replicon chromosome
Accession NZ_CP032739
Organism Enterococcus casseliflavus strain EC-369

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag D8N35_RS16030 Protein ID WP_121261993.1
Coordinates 3267159..3267509 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag D8N35_RS16035 Protein ID WP_205589237.1
Coordinates 3267503..3267793 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8N35_RS16000 3262822..3264183 - 1362 WP_005225442.1 hypothetical protein -
D8N35_RS16005 3264533..3265456 - 924 WP_121261987.1 peptide-methionine (S)-S-oxide reductase MsrA -
D8N35_RS16015 3265771..3266316 - 546 WP_205589236.1 isochorismatase family protein -
D8N35_RS16020 3266419..3266844 + 426 WP_121261991.1 AsnC family transcriptional regulator -
D8N35_RS16030 3267159..3267509 - 351 WP_121261993.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D8N35_RS16035 3267503..3267793 - 291 WP_205589237.1 PbsX family transcriptional regulator Antitoxin
D8N35_RS16040 3268052..3268219 - 168 WP_121261995.1 PTS glucose transporter subunit IIA -
D8N35_RS16045 3268559..3268657 - 99 WP_121261997.1 type I toxin-antitoxin system Fst family toxin -
D8N35_RS16050 3268745..3269503 - 759 WP_121261999.1 type I methionyl aminopeptidase -
D8N35_RS16055 3269546..3270283 - 738 WP_121262001.1 tRNA pseudouridine(38-40) synthase TruA -
D8N35_RS18325 3270348..3270494 - 147 WP_205589238.1 hypothetical protein -
D8N35_RS18330 3270540..3271439 - 900 WP_205589239.1 EVE domain-containing protein -
D8N35_RS16070 3271649..3272128 - 480 WP_008379108.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(13-96)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13039.01 Da        Isoelectric Point: 7.3540

>T112199 WP_121261993.1 NZ_CP032739:c3267509-3267159 [Enterococcus casseliflavus]
MVRKPRQGDILLLDTAPRSGHEQTGKRPYIVLSHDMIADYSNVATVAPISSTSRNYPLYVSIDPDYGMKTTGKVLLDQLT
TIDYEASACVFLEKAHEHLVDELLMKVRTVFQKVSK

Download         Length: 351 bp

>T112199 NZ_CP032739:c3267509-3267159 [Enterococcus casseliflavus]
ATGGTAAGAAAACCTAGACAAGGGGATATTTTGTTATTGGATACAGCTCCTCGTTCTGGACATGAACAAACGGGAAAAAG
ACCCTATATTGTTTTAAGTCATGACATGATTGCAGATTATAGTAACGTTGCCACCGTTGCTCCGATTTCTAGTACAAGTA
GAAACTATCCGTTATATGTAAGTATTGATCCAGACTATGGAATGAAGACAACAGGAAAAGTATTGTTGGATCAATTAACG
ACAATTGATTATGAAGCCAGCGCGTGTGTTTTTTTGGAAAAAGCGCATGAGCACTTGGTTGATGAGTTATTGATGAAAGT
TAGAACAGTATTTCAAAAAGTAAGTAAATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 11277.00 Da        Isoelectric Point: 4.9389

>AT112199 WP_205589237.1 NZ_CP032739:c3267793-3267503 [Enterococcus casseliflavus]
MYIQKEVLLMEMLPIKKWGNSNGLRLPKYIMEYLGIHTDDKVKIIQEEVNGHKRLIIEAANLENELTIEELFENYTAEKN
HVEIQHLGEAVGNEKW

Download         Length: 291 bp

>AT112199 NZ_CP032739:c3267793-3267503 [Enterococcus casseliflavus]
ATGTATATACAGAAAGAGGTGTTGCTCATGGAAATGCTACCTATAAAAAAATGGGGCAATAGTAACGGTCTTAGATTGCC
AAAGTACATTATGGAGTATCTTGGCATTCATACTGATGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGACATAAAC
GGCTAATCATCGAAGCAGCAAATTTAGAAAACGAGTTAACGATTGAAGAGTTATTTGAAAATTACACTGCTGAGAAAAAT
CATGTGGAAATTCAACATTTAGGTGAAGCAGTAGGGAATGAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References