Detailed information of TA system TA112179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 129093..130123
• Replicon: plasmid unnamed2
• Accession: NZ_CP032704
• Organism: Pantoea dispersa strain DSM 32899

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: D8B20_RS20615
• Protein ID: WP_145891832.1
• Coordinates: 129548..130123 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: D8B20_RS20610
• Protein ID: WP_145891830.1
• Coordinates: 129093..129551 (+)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8B20_RS20585	124291..125361	+	1071	WP_145891824.1	catabolic alanine racemase DadX	-
D8B20_RS20590	125378..125671	+	294	WP_145891826.1	hypothetical protein	-
D8B20_RS20595	125719..126315	-	597	WP_145891828.1	short chain dehydrogenase	-
D8B20_RS20600	126436..127305	+	870	WP_145892060.1	LysR family transcriptional regulator	-
D8B20_RS20605	127577..128724	+	1148	Protein_112	IS3 family transposase	-
D8B20_RS20610	129093..129551	+	459	WP_145891830.1	excisionase family DNA-binding protein	Antitoxin
D8B20_RS20615	129548..130123	+	576	WP_145891832.1	PIN domain-containing protein	Toxin
D8B20_RS20620	130173..131210	-	1038	WP_145891834.1	tyrosine-type recombinase/integrase	-
D8B20_RS20625	132851..133642	-	792	WP_145891836.1	alpha/beta hydrolase	-
D8B20_RS20630	133834..134742	-	909	WP_145891838.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..275665	275665
	-	flank	IS/Tn	-	-	127852..128724	872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (81-119)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21573.68 Da
• Isoelectric Point: 4.6350

>T112179 WP_145891832.1 NZ_CP032704:129548-130123 [Pantoea dispersa]
MSSGYTVVFDACVVYPAPLRSFLMYLAAGGQFCARWSEDIHEEWIRNVLLNRPELSRTQLERVRALMDRHVPDALVTGYQ
SLVESIRGLPDEDDRHVVAAAIVTQAEGIVTFNLRDFPDEVLSVWNLRAIHPDSFIADLTDLDITTVIDAARRQRASLLS
PPFTPDEFLDCLLRQQLPETVSRLRPLAALI

Nucleotide

• Length: 576 bp

>T112179 NZ_CP032704:129548-130123 [Pantoea dispersa]
ATGAGTTCAGGTTACACCGTAGTATTTGACGCTTGCGTTGTTTACCCTGCACCTCTGCGCAGTTTCCTGATGTACCTTGC
TGCCGGCGGGCAATTTTGCGCGCGATGGAGTGAAGACATCCATGAAGAGTGGATCCGTAATGTATTGCTCAATCGCCCCG
AGCTTAGCCGGACACAGCTGGAACGTGTTCGCGCATTAATGGATCGCCACGTACCGGATGCGCTGGTAACCGGATATCAG
TCTCTGGTTGAATCCATCAGGGGACTGCCTGATGAAGACGACCGTCACGTCGTTGCCGCTGCGATTGTTACCCAGGCTGA
AGGTATTGTGACCTTTAACCTCAGAGATTTTCCTGATGAGGTATTGTCGGTCTGGAACCTGCGTGCCATTCACCCTGACA
GCTTTATCGCTGATCTAACTGATCTGGACATCACTACTGTGATTGACGCGGCCCGGCGCCAGCGCGCCAGTCTTTTGTCT
CCGCCTTTCACGCCCGATGAGTTTCTCGATTGCCTGCTGCGACAGCAGCTTCCGGAAACCGTCTCCCGGTTACGACCTCT
TGCCGCATTAATCTGA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 17032.41 Da
• Isoelectric Point: 5.7125

>AT112179 WP_145891830.1 NZ_CP032704:129093-129551 [Pantoea dispersa]
MTQLQNKLPSLQEAELARLCSRELSAVLETRAESQQVSLTDKRGQIHNVSIPVSALRMLVDVLTELGEGNAVRLVPVGAE
MTTQEVADLLNISRPTLIRFLDNGELPYHRTGNRRKIRYVDALAYRNKLVNQRHDALDELGALDQELGLGYE

Nucleotide

• Length: 459 bp

>AT112179 NZ_CP032704:129093-129551 [Pantoea dispersa]
ATGACACAGCTTCAGAATAAGCTTCCTTCCCTCCAGGAAGCGGAACTTGCGCGCCTGTGCAGCCGGGAACTCTCCGCCGT
GCTCGAAACACGCGCAGAGTCACAACAGGTTTCACTCACTGACAAACGTGGGCAGATCCATAATGTCAGCATTCCGGTTA
GCGCGCTGCGTATGCTGGTAGATGTACTCACCGAGTTGGGTGAGGGAAATGCCGTGCGCCTTGTTCCGGTCGGTGCAGAG
ATGACAACGCAGGAAGTGGCCGATCTGCTTAACATTTCGCGTCCGACACTGATTCGTTTTCTGGATAATGGTGAACTGCC
TTACCATCGTACAGGTAACCGTCGTAAAATCCGCTACGTGGACGCACTTGCATACCGCAACAAACTGGTAAATCAGCGGC
ATGATGCGCTTGATGAGCTCGGCGCGCTCGATCAGGAACTTGGGCTGGGTTACGAATGA