Detailed information of TA system TA112175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 774943..776051
• Replicon: chromosome
• Accession: NZ_CP032700
• Organism: Streptococcus pyogenes strain TSPY556

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: D8S77_RS04000
• Protein ID: WP_000233000.1
• Coordinates: 775182..776051 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: D8S77_RS03995
• Protein ID: WP_000205227.1
• Coordinates: 774943..775167 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8S77_RS03970	770057..771685	+	1629	WP_023078867.1	AAA family ATPase	-
D8S77_RS03975	771678..772010	+	333	Protein_705	UvrD-helicase domain-containing protein	-
D8S77_RS09355	772322..772486	+	165	Protein_706	DNA recombinase	-
D8S77_RS03980	772523..772819	+	297	WP_002360685.1	hypothetical protein	-
D8S77_RS03985	772820..773236	+	417	WP_000323438.1	recombinase	-
D8S77_RS03990	773256..774800	+	1545	WP_129322392.1	recombinase family protein	-
D8S77_RS03995	774943..775167	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
D8S77_RS04000	775182..776051	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
D8S77_RS04005	776032..776766	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
D8S77_RS04010	776799..777707	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
D8S77_RS04015	777704..778246	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
D8S77_RS04020	778339..779133	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
D8S77_RS04030	779409..779981	+	573	WP_164976498.1	HTH domain-containing protein	-
D8S77_RS04040	779954..780361	+	408	WP_072387101.1	WYL domain-containing protein	-
D8S77_RS09275	780472..780834	+	363	Protein_718	riboflavin biosynthesis protein RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / aph(3')-III / tet(M)	speC / mf2 / grab	659406..867494	208088
	-	flank	IS/Tn	ant(6)-Ia / aph(3')-III / tet(M)	-	776799..787608	10809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T112175 WP_000233000.1 NZ_CP032700:775182-776051 [Streptococcus pyogenes]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T112175 NZ_CP032700:775182-776051 [Streptococcus pyogenes]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT112175 WP_000205227.1 NZ_CP032700:774943-775167 [Streptococcus pyogenes]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT112175 NZ_CP032700:774943-775167 [Streptococcus pyogenes]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1