Detailed information of TA system TA112171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 417878..418689
• Replicon: plasmid pRCCGE525b
• Accession: NZ_CP032696
• Organism: Rhizobium jaguaris strain CCGE525

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: CCGE525_RS36155
• Protein ID: WP_120709078.1
• Coordinates: 418153..418689 (+)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: CCGE525_RS36150
• Protein ID: WP_120709203.1
• Coordinates: 417878..418156 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE525_RS36130	413780..414775	+	996	WP_120709076.1	helix-turn-helix transcriptional regulator	-
CCGE525_RS36135	415056..416566	-	1511	Protein_375	ISL3 family transposase	-
CCGE525_RS36140	416812..417083	-	272	Protein_376	hypothetical protein	-
CCGE525_RS36145	417294..417476	-	183	WP_120709077.1	hypothetical protein	-
CCGE525_RS36150	417878..418156	+	279	WP_120709203.1	DUF1778 domain-containing protein	Antitoxin
CCGE525_RS36155	418153..418689	+	537	WP_120709078.1	GNAT family N-acetyltransferase	Toxin
CCGE525_RS38850	418822..419082	+	261	WP_205587533.1	hypothetical protein	-
CCGE525_RS36165	419289..419816	-	528	WP_162950435.1	universal stress protein	-
CCGE525_RS36170	420056..421510	-	1455	WP_120709080.1	GntP family permease	-
CCGE525_RS36175	421514..422554	-	1041	WP_120709081.1	patatin-like phospholipase family protein	-
CCGE525_RS36190	423113..423268	+	156	WP_205587539.1	hypothetical protein	-
CCGE525_RS36195	423427..423669	+	243	WP_120709082.1	type II toxin-antitoxin system VapB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..550563	550563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (14-86)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19305.95 Da
• Isoelectric Point: 7.3197

>T112171 WP_120709078.1 NZ_CP032696:418153-418689 [Rhizobium jaguaris]
MTVPDWHEEPIAKGHDRKGFDCGQADLNDFLARYARQAHESGASKTYVAVGNTDNKTILGFYTLSPAQVDFDLAPETARP
SGGGRHPIGGFRLGRLAVSTSYQGRGLGGELLIAAARRCIQVSSQVGGSLLLIDAKDERVAEWYKSYGALEIPKLRLSLV
LPYSVFIDVMEQAGKPIL

Nucleotide

• Length: 537 bp

>T112171 NZ_CP032696:418153-418689 [Rhizobium jaguaris]
ATGACTGTTCCGGACTGGCATGAAGAGCCGATCGCCAAAGGGCATGATCGCAAGGGCTTCGATTGTGGTCAGGCCGATCT
CAACGACTTTCTGGCGAGGTATGCCCGACAGGCTCATGAGAGCGGCGCCTCAAAGACCTACGTGGCGGTCGGCAATACGG
ACAACAAGACGATTCTCGGTTTCTACACACTGAGCCCGGCCCAGGTAGACTTTGACCTGGCGCCCGAGACGGCACGTCCA
TCCGGTGGCGGACGACATCCGATCGGTGGGTTTCGCCTCGGCCGCCTGGCGGTCAGCACATCTTATCAGGGCCGAGGTCT
TGGCGGCGAATTGTTGATTGCCGCTGCCCGGCGATGCATCCAGGTTTCATCACAGGTCGGTGGTTCGCTGCTGCTGATTG
ACGCCAAGGATGAACGAGTCGCGGAGTGGTACAAGTCCTATGGCGCGCTCGAAATTCCCAAGTTGCGTCTTTCTCTCGTT
CTGCCCTATTCCGTTTTCATCGATGTGATGGAGCAGGCGGGTAAACCGATCCTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.03 Da
• Isoelectric Point: 10.7495

>AT112171 WP_120709203.1 NZ_CP032696:417878-418156 [Rhizobium jaguaris]
MPRAAAENTNRFPMQIPPAEKARLMRAAALERTSLKEFVLRNALVAAEAVIEKAERISLDEEQTRFILDLMDNPPQPNAK
LKAAARLLAARK

Nucleotide

• Length: 279 bp

>AT112171 NZ_CP032696:417878-418156 [Rhizobium jaguaris]
ATGCCCAGAGCCGCAGCTGAGAATACGAACCGCTTCCCCATGCAGATTCCGCCAGCCGAGAAAGCGCGGCTGATGCGCGC
GGCTGCGCTTGAGCGCACGAGCCTGAAAGAGTTCGTATTGCGCAACGCGCTTGTGGCGGCGGAAGCCGTCATCGAGAAAG
CCGAGCGGATCAGCCTGGATGAAGAACAGACCCGCTTCATACTGGATCTGATGGATAACCCGCCACAGCCGAACGCAAAA
CTGAAAGCAGCAGCTCGCTTGCTCGCTGCCCGCAAATGA