Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 6451..6995 Replicon plasmid pRCCGE525b
Accession NZ_CP032696
Organism Rhizobium jaguaris strain CCGE525

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag CCGE525_RS34205 Protein ID WP_120708797.1
Coordinates 6451..6774 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag CCGE525_RS34210 Protein ID WP_120708798.1
Coordinates 6771..6995 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCGE525_RS34170 1922..2141 - 220 Protein_2 hypothetical protein -
CCGE525_RS34175 2209..3024 - 816 WP_120708792.1 calcium-binding protein -
CCGE525_RS38815 4036..4395 + 360 WP_205587534.1 hypothetical protein -
CCGE525_RS34190 4500..4706 + 207 WP_120708794.1 cold-shock protein -
CCGE525_RS34195 4837..6012 - 1176 WP_120708795.1 site-specific integrase -
CCGE525_RS34200 6052..6339 - 288 WP_120708796.1 hypothetical protein -
CCGE525_RS34205 6451..6774 - 324 WP_120708797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CCGE525_RS34210 6771..6995 - 225 WP_120708798.1 antitoxin MazE family protein Antitoxin
CCGE525_RS34215 7548..7856 - 309 WP_120708799.1 hypothetical protein -
CCGE525_RS34220 7986..8492 + 507 WP_120708800.1 HIT family protein -
CCGE525_RS34225 8679..9548 + 870 WP_120708801.1 hypothetical protein -
CCGE525_RS34230 9643..11376 - 1734 WP_120708802.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..550563 550563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

Antitoxin MazE-like

(6-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11330.20 Da        Isoelectric Point: 10.7342

>T112170 WP_120708797.1 NZ_CP032696:c6774-6451 [Rhizobium jaguaris]
VRRGDIVTVAISGDFGKPRPALIIQTDQFDATGTVTVLLISGTLVDAPLIRPTIQPTAQSGLRKASQVMVDKAMSVKRDR
IGTTIGRLEDDSLLAVTRSLAVFLGVA

Download         Length: 324 bp

>T112170 NZ_CP032696:c6774-6451 [Rhizobium jaguaris]
GTGAGGCGCGGTGACATCGTGACCGTCGCAATCAGCGGCGATTTCGGCAAGCCTCGTCCAGCACTAATCATCCAGACCGA
TCAGTTTGATGCAACGGGAACAGTCACTGTCCTGCTGATTTCCGGCACACTCGTCGACGCGCCGCTCATTCGACCAACAA
TTCAGCCAACCGCACAAAGCGGTTTGCGCAAAGCCTCGCAAGTGATGGTGGACAAAGCCATGTCCGTGAAACGGGATAGA
ATTGGCACGACGATCGGTCGCCTGGAAGATGATTCCCTCCTGGCGGTCACAAGGTCGCTCGCCGTTTTTTTGGGTGTCGC
CTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8168.14 Da        Isoelectric Point: 5.6546

>AT112170 WP_120708798.1 NZ_CP032696:c6995-6771 [Rhizobium jaguaris]
MASSVNERVQKRRETLRAAGLRPVQIWVPDTRRPGFAAECHRQSVQVALADVADRGLQDFLDAALGDLDHEGEA

Download         Length: 225 bp

>AT112170 NZ_CP032696:c6995-6771 [Rhizobium jaguaris]
ATGGCAAGCTCGGTCAACGAAAGAGTTCAAAAGCGCCGTGAAACGTTGCGGGCCGCTGGGCTGCGTCCCGTGCAAATATG
GGTTCCTGACACCCGTCGCCCTGGTTTTGCCGCCGAATGCCATCGTCAGTCCGTTCAGGTCGCCCTTGCCGATGTCGCCG
ACCGCGGTCTGCAAGATTTCCTTGACGCCGCTCTCGGTGACCTCGACCACGAGGGTGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References