Detailed information of TA system TA112168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1039171..1039961
• Replicon: plasmid pRCCGE525c
• Accession: NZ_CP032695
• Organism: Rhizobium jaguaris strain CCGE525

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: CCGE525_RS27120
• Protein ID: WP_120707366.1
• Coordinates: 1039464..1039961 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: CCGE525_RS27115
• Protein ID: WP_120707365.1
• Coordinates: 1039171..1039467 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE525_RS27085	1034567..1034818	+	252	WP_120707360.1	type II toxin-antitoxin system VapB family antitoxin	-
CCGE525_RS27090	1034969..1035742	-	774	WP_120707361.1	site-2 protease family protein	-
CCGE525_RS27095	1036227..1037213	+	987	WP_162950323.1	hypothetical protein	-
CCGE525_RS27100	1037299..1037751	+	453	WP_120708649.1	hypothetical protein	-
CCGE525_RS38510	1038032..1038583	+	552	WP_162950324.1	sel1 repeat family protein	-
CCGE525_RS27110	1038808..1039026	-	219	WP_120707364.1	2OG-Fe(II) oxygenase	-
CCGE525_RS27115	1039171..1039467	+	297	WP_120707365.1	DUF1778 domain-containing protein	Antitoxin
CCGE525_RS27120	1039464..1039961	+	498	WP_120707366.1	GNAT family N-acetyltransferase	Toxin
CCGE525_RS27130	1040584..1041666	-	1083	WP_120707367.1	sensor domain-containing diguanylate cyclase	-
CCGE525_RS27135	1041725..1042039	-	315	WP_162950325.1	EAL domain-containing protein	-
CCGE525_RS27140	1042377..1043660	+	1284	WP_120707369.1	cystathionine gamma-synthase family protein	-
CCGE525_RS27145	1043698..1044843	-	1146	WP_120707370.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tssC-5 / katA	1..2584926	2584926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17754.32 Da
• Isoelectric Point: 7.4230

>T112168 WP_120707366.1 NZ_CP032695:1039464-1039961 [Rhizobium jaguaris]
VTPSAPAPLADHHELAEFSSGVLELDDWLRRRARANQAGGASRTFVVCEGDRVIAYYALASGAVKQPEAPGRFRRNMPDP
IPVAVLGRLAIDQSFQGRGIGRALVRDAGLRLLNAAEILGIRGVMVHAISDDARAFYEAVGFLSSPSDPMMLMVGLHDLH
SALNR

Nucleotide

• Length: 498 bp

>T112168 NZ_CP032695:1039464-1039961 [Rhizobium jaguaris]
GTGACGCCCAGCGCACCGGCGCCGTTGGCCGATCATCACGAACTCGCCGAATTCAGTTCCGGCGTGCTTGAACTGGACGA
CTGGCTGCGCCGCCGTGCCCGGGCCAATCAGGCGGGCGGCGCGTCGCGCACCTTCGTTGTGTGCGAGGGAGACCGCGTCA
TCGCGTATTACGCGCTTGCGTCCGGGGCGGTGAAGCAGCCGGAAGCACCCGGACGTTTTCGGCGCAATATGCCCGATCCG
ATTCCGGTCGCCGTGCTTGGCCGCCTCGCCATTGATCAGTCCTTTCAGGGGCGCGGTATTGGCAGGGCATTGGTGCGCGA
CGCAGGCCTCCGTCTGCTCAACGCGGCCGAAATCCTCGGAATTCGCGGCGTCATGGTGCATGCGATTTCCGATGACGCAC
GGGCCTTCTATGAAGCGGTTGGCTTCCTGTCTTCTCCGTCCGATCCCATGATGCTAATGGTCGGGTTGCATGACCTCCAC
AGCGCGCTGAATCGTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11167.75 Da
• Isoelectric Point: 7.0154

>AT112168 WP_120707365.1 NZ_CP032695:1039171-1039467 [Rhizobium jaguaris]
MPTSHTNKSPDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIFTVSPEIYAEYLARLDAPPQ
PNERLKRTMSTKAPWDDV

Nucleotide

• Length: 297 bp

>AT112168 NZ_CP032695:1039171-1039467 [Rhizobium jaguaris]
ATGCCCACCTCGCACACGAACAAAAGCCCGGACGCCAGCGTGCCGCTGAATATGCGCATCAAGCCTGCGACCCGCAATCT
GATCGACCGCGCCGCTGAGTTGCTTGGTAAGACACGCACGGATTTCATGCTTGAAGCTTCTGAGCGCCGCGCTGAGGAGG
TGCTGCTCGACCGTACCATATTCACGGTCAGCCCCGAAATCTACGCCGAATACCTTGCCCGCCTCGACGCACCCCCGCAG
CCCAATGAGCGTTTGAAGCGCACTATGTCGACCAAAGCGCCGTGGGATGACGTGTGA