Detailed information of TA system TA112166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 898481..899157
• Replicon: plasmid pRCCGE525c
• Accession: NZ_CP032695
• Organism: Rhizobium jaguaris strain CCGE525

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCGE525_RS26410
• Protein ID: WP_120707259.1
• Coordinates: 898481..898903 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCGE525_RS26415
• Protein ID: WP_120707260.1
• Coordinates: 898900..899157 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE525_RS26375	893709..894061	-	353	Protein_828	Tn3 family transposase	-
CCGE525_RS26390	895080..895898	+	819	WP_120707255.1	transporter substrate-binding domain-containing protein	-
CCGE525_RS26395	896014..896661	+	648	WP_120707256.1	amino acid ABC transporter permease	-
CCGE525_RS26400	896661..897323	+	663	WP_120707257.1	amino acid ABC transporter permease	-
CCGE525_RS26405	897325..898101	+	777	WP_120707258.1	amino acid ABC transporter ATP-binding protein	-
CCGE525_RS26410	898481..898903	-	423	WP_120707259.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCGE525_RS26415	898900..899157	-	258	WP_120707260.1	Arc family DNA-binding protein	Antitoxin
CCGE525_RS26420	899912..901411	-	1500	WP_120707261.1	aminotransferase class V-fold PLP-dependent enzyme	-
CCGE525_RS26425	901508..901990	+	483	WP_120707262.1	Lrp/AsnC family transcriptional regulator	-
CCGE525_RS26435	902436..902702	+	267	WP_120707264.1	DUF2277 domain-containing protein	-
CCGE525_RS26440	903086..904126	+	1041	WP_120707265.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tssC-5 / katA	1..2584926	2584926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15307.46 Da
• Isoelectric Point: 4.5386

>T112166 WP_120707259.1 NZ_CP032695:c898903-898481 [Rhizobium jaguaris]
MIVLDTNVISELWKVEPDTNVLAWIDAQLIETLYLSTITVAELRYGVATMPEGKRQTIYQDRLEREVLPAFSGRVLSFDL
DASQAYAELMARAKEAGRAIGKADGYIAATAAARGLMVATRDTSPFQAAGLNVINPWNSS

Nucleotide

• Length: 423 bp

>T112166 NZ_CP032695:c898903-898481 [Rhizobium jaguaris]
ATGATCGTTCTCGATACCAATGTGATCTCCGAACTCTGGAAAGTCGAGCCGGATACGAACGTTTTGGCCTGGATTGACGC
CCAACTGATCGAAACCCTCTACCTGTCTACGATCACCGTCGCTGAATTGCGCTATGGTGTGGCAACGATGCCGGAAGGCA
AACGGCAAACGATCTACCAGGATCGTCTTGAACGGGAGGTTTTGCCAGCGTTCTCCGGCCGAGTTCTTTCTTTCGACCTG
GATGCGTCGCAAGCCTACGCCGAGCTAATGGCGCGAGCGAAGGAAGCCGGTAGGGCTATTGGGAAGGCTGATGGCTACAT
CGCCGCAACGGCCGCGGCGCGCGGTCTGATGGTTGCCACACGTGACACAAGTCCCTTCCAGGCCGCAGGTCTGAACGTCA
TTAATCCCTGGAATTCCTCGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9328.64 Da
• Isoelectric Point: 7.2065

>AT112166 WP_120707260.1 NZ_CP032695:c899157-898900 [Rhizobium jaguaris]
MPSITIRNVPDEVHRAIRVRAALHGRSAEAEIRSILEQAAKPEGRLKLGTLLTSIAREAGGLTDAEAERLNQLRDKTPAE
PMSFE

Nucleotide

• Length: 258 bp

>AT112166 NZ_CP032695:c899157-898900 [Rhizobium jaguaris]
ATGCCGTCCATCACTATTCGCAACGTGCCAGACGAAGTCCATCGTGCCATTCGTGTCCGGGCCGCCCTGCACGGCCGCAG
CGCCGAGGCAGAAATCCGCAGCATTCTCGAACAGGCTGCCAAACCAGAGGGACGTTTGAAGCTGGGCACACTATTGACCT
CTATCGCCCGTGAGGCTGGAGGTCTGACGGACGCCGAAGCGGAACGCCTCAATCAGCTTCGTGACAAGACTCCGGCTGAA
CCTATGAGCTTTGAATGA