Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 853328..853902 Replicon plasmid pRCCGE525c
Accession NZ_CP032695
Organism Rhizobium jaguaris strain CCGE525

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CCGE525_RS26185 Protein ID WP_120707224.1
Coordinates 853537..853902 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CCGE525_RS26180 Protein ID WP_120708636.1
Coordinates 853328..853543 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCGE525_RS26160 848901..849368 + 468 WP_120708634.1 cytochrome P460 family protein -
CCGE525_RS26165 849378..850064 + 687 WP_120707222.1 DUF4405 domain-containing protein -
CCGE525_RS26170 850107..851252 + 1146 WP_120707223.1 aldo/keto reductase -
CCGE525_RS26175 851815..853038 + 1224 WP_120708635.1 ATP-binding protein -
CCGE525_RS26180 853328..853543 + 216 WP_120708636.1 ribbon-helix-helix domain-containing protein Antitoxin
CCGE525_RS26185 853537..853902 + 366 WP_120707224.1 type II toxin-antitoxin system VapC family toxin Toxin
CCGE525_RS26190 854177..855304 + 1128 WP_120707225.1 acyl-CoA dehydrogenase family protein -
CCGE525_RS26195 855369..856601 + 1233 WP_120707226.1 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) subunit alpha -
CCGE525_RS26200 856604..857617 + 1014 WP_120707227.1 alpha-ketoacid dehydrogenase subunit beta -
CCGE525_RS26205 857621..858847 + 1227 WP_120707228.1 2-oxo acid dehydrogenase subunit E2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - tssC-5 / katA 1..2584926 2584926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13635.99 Da        Isoelectric Point: 7.2028

>T112165 WP_120707224.1 NZ_CP032695:853537-853902 [Rhizobium jaguaris]
MVKSLFDTNVLIDYLNAVPQARDELTKYREKAISIITWMEVLVGAKAEVAIGTRAFLAGFAVIAVDDAIAERAVSLRQLH
RIKLPDAIIWATANVHSMLLITRNTKDFPREMPDIRVPYEI

Download         Length: 366 bp

>T112165 NZ_CP032695:853537-853902 [Rhizobium jaguaris]
ATGGTAAAGTCTCTCTTCGACACCAATGTCTTGATCGACTATCTCAATGCCGTACCGCAAGCGCGAGACGAGCTGACAAA
ATATCGCGAAAAGGCGATAAGCATCATTACCTGGATGGAGGTTCTCGTTGGTGCCAAGGCTGAGGTGGCAATCGGAACGC
GCGCTTTCCTGGCAGGCTTTGCAGTCATCGCTGTCGACGACGCGATAGCAGAACGAGCGGTATCTCTGCGACAACTGCAT
CGTATCAAGCTTCCAGATGCTATTATCTGGGCAACGGCCAATGTCCACTCGATGTTGCTGATCACCCGAAACACGAAGGA
TTTCCCGCGGGAAATGCCGGACATTCGGGTGCCTTACGAAATCTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8109.16 Da        Isoelectric Point: 4.7872

>AT112165 WP_120708636.1 NZ_CP032695:853328-853543 [Rhizobium jaguaris]
MRTLVDIGDPEVKALDRLAQREKMSRAALIRKAIDDFLARNNADSEAEAFGLWGDRKIDGLAYQENMRGEW

Download         Length: 216 bp

>AT112165 NZ_CP032695:853328-853543 [Rhizobium jaguaris]
ATGAGAACGTTGGTGGATATCGGCGACCCGGAAGTCAAAGCGCTGGACCGGCTGGCACAGCGAGAAAAAATGTCGCGGGC
GGCACTGATTCGCAAGGCGATTGATGACTTTCTGGCCAGGAACAACGCCGATAGTGAAGCAGAGGCATTCGGGCTCTGGG
GTGATCGAAAGATCGATGGTTTGGCGTATCAGGAAAACATGCGCGGCGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References