Detailed information of TA system TA112158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 454049..454593
• Replicon: plasmid pRCCGE532b
• Accession: NZ_CP032692
• Organism: Rhizobium sp. CCGE532

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: N6V6D9
• Locus tag: CCGE532_RS31565
• Protein ID: WP_004119734.1
• Coordinates: 454270..454593 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CCGE532_RS31560
• Protein ID: WP_120764323.1
• Coordinates: 454049..454273 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE532_RS31545	450123..450294	+	172	Protein_418	IS5/IS1182 family transposase	-
CCGE532_RS31550	451249..452499	+	1251	WP_120764322.1	MFS transporter	-
CCGE532_RS31555	452748..453652	+	905	Protein_420	hypothetical protein	-
CCGE532_RS31560	454049..454273	+	225	WP_120764323.1	antitoxin MazE family protein	Antitoxin
CCGE532_RS31565	454270..454593	+	324	WP_004119734.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CCGE532_RS31570	454668..455054	-	387	Protein_423	hypothetical protein	-
CCGE532_RS31575	455133..455768	-	636	WP_120764324.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
CCGE532_RS33715	456383..456592	-	210	WP_135488292.1	hypothetical protein	-
CCGE532_RS31590	456780..457078	-	299	Protein_426	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..506160	506160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11356.28 Da
• Isoelectric Point: 10.7342

>T112158 WP_004119734.1 NZ_CP032692:454270-454593 [Rhizobium sp. CCGE532]
VRRGDIVTVAISGDFGKPRPALIIQADQFDATGTITVLLISSTLVDAPLIRPTIQPTVQSGLRKASQVMVDKAMSVKRDR
IGTTIGRLEDDALLAVTRSLAVFLGVA

Nucleotide

• Length: 324 bp

>T112158 NZ_CP032692:454270-454593 [Rhizobium sp. CCGE532]
GTGAGGCGCGGTGACATTGTGACCGTCGCAATCAGCGGTGATTTCGGCAAGCCGCGTCCAGCACTAATCATCCAGGCCGA
TCAGTTTGATGCAACTGGAACGATCACTGTCCTGCTGATCTCCAGCACACTGGTCGACGCGCCGCTCATTCGACCAACCA
TTCAGCCGACAGTGCAAAGCGGATTGCGAAAAGCCTCGCAAGTGATGGTGGACAAAGCCATGTCCGTGAAACGGGATAGA
ATTGGCACAACGATCGGTCGCCTGGAAGATGATGCGCTCCTGGCGGTCACAAGATCGCTCGCCGTTTTTTTAGGTGTTGC
TTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8117.00 Da
• Isoelectric Point: 4.3939

>AT112158 WP_120764323.1 NZ_CP032692:454049-454273 [Rhizobium sp. CCGE532]
MASSVNERVQKRRETLRAAGLRPVQIWVPDTRRPGFAAECHRQSVQVALADAADPGLQDFLDAALDDLDDEGEA

Nucleotide

• Length: 225 bp

>AT112158 NZ_CP032692:454049-454273 [Rhizobium sp. CCGE532]
ATGGCAAGCTCGGTCAACGAACGAGTTCAAAAGCGCCGTGAGACGTTGCGCGCCGCTGGGCTGCGTCCTGTGCAAATATG
GGTTCCCGATACTCGTCGCCCTGGTTTTGCCGCCGAATGCCATCGTCAGTCCGTTCAGGTAGCCCTTGCCGATGCCGCCG
ATCCCGGTCTGCAAGACTTCCTTGACGCCGCTCTCGATGACCTGGACGACGAGGGTGAAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A5F1S1T4