Detailed information of TA system TA112157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 170536..171119
• Replicon: plasmid pRCCGE532c
• Accession: NZ_CP032691
• Organism: Rhizobium sp. CCGE532

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCGE532_RS26580
• Protein ID: WP_120669685.1
• Coordinates: 170536..170922 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCGE532_RS26585
• Protein ID: WP_120669687.1
• Coordinates: 170919..171119 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE532_RS26580	170536..170922	-	387	WP_120669685.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCGE532_RS26585	170919..171119	-	201	WP_120669687.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CCGE532_RS26590	171279..173056	-	1778	Protein_140	ABC transporter ATP-binding protein	-
CCGE532_RS26595	173064..173461	-	398	Protein_141	glycosyltransferase family 2 protein	-
CCGE532_RS26600	173463..174172	-	710	Protein_142	glycosyltransferase family 2 protein	-
CCGE532_RS26605	174454..174705	+	252	WP_120669689.1	transcriptional regulator	-
CCGE532_RS26610	174705..175067	+	363	WP_120669690.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..747941	747941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14118.24 Da
• Isoelectric Point: 6.7690

>T112157 WP_120669685.1 NZ_CP032691:c170922-170536 [Rhizobium sp. CCGE532]
VILADTSIWIDHFRSADTELRRIIEDDRLLCHPVVIGELALGSLRDRGDVIAFLSAQRGAVVATHDEVMIMIDRHGIFSM
GIGYTDAHLLASVLLDHKASLWTRDKRLRAAADKAGASLHIPVNAPHH

Nucleotide

• Length: 387 bp

>T112157 NZ_CP032691:c170922-170536 [Rhizobium sp. CCGE532]
GTGATACTTGCAGACACCTCGATCTGGATTGATCATTTCCGATCTGCCGATACAGAGCTGCGCAGGATTATCGAGGATGA
TCGTCTGCTCTGCCATCCGGTTGTGATTGGCGAGCTGGCGCTTGGCAGCCTCCGCGATCGCGGTGACGTGATAGCTTTCC
TATCGGCCCAGCGCGGTGCGGTCGTCGCAACGCATGACGAAGTCATGATAATGATCGATCGACATGGTATTTTCAGCATG
GGCATTGGCTACACGGATGCTCACTTGCTGGCGTCTGTTCTTCTCGACCACAAAGCGTCCTTATGGACCAGAGACAAGCG
CCTGCGGGCAGCGGCCGACAAGGCAGGAGCCTCACTGCATATACCGGTCAACGCGCCTCATCACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7090.24 Da
• Isoelectric Point: 11.1107

>AT112157 WP_120669687.1 NZ_CP032691:c171119-170919 [Rhizobium sp. CCGE532]
MRSTINLDDALLDRAKSLTGIKETAALVRQALETLVRVESGKRLIALGGTMPEAEAAPRRRSTAAK

Nucleotide

• Length: 201 bp

>AT112157 NZ_CP032691:c171119-170919 [Rhizobium sp. CCGE532]
ATGCGATCTACTATCAATCTCGACGATGCACTCTTGGATAGGGCGAAATCCTTGACTGGGATCAAGGAAACTGCGGCGCT
CGTTCGCCAGGCATTGGAGACGCTTGTTCGCGTAGAGTCCGGAAAACGCCTCATCGCGCTTGGAGGAACCATGCCTGAGG
CAGAGGCAGCTCCGCGCCGCCGGAGCACAGCGGCTAAGTGA