Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 473767..474341 Replicon plasmid pRCCGE532d
Accession NZ_CP032690
Organism Rhizobium sp. CCGE532

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CCGE532_RS21200 Protein ID WP_120667679.1
Coordinates 473976..474341 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CCGE532_RS21195 Protein ID WP_120669292.1
Coordinates 473767..473982 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCGE532_RS21180 469821..471767 - 1947 WP_120667675.1 ABC transporter substrate-binding protein -
CCGE532_RS21185 471978..472691 + 714 WP_120669290.1 FadR family transcriptional regulator -
CCGE532_RS21190 472780..473700 + 921 WP_120667677.1 dihydrodipicolinate synthase family protein -
CCGE532_RS21195 473767..473982 + 216 WP_120669292.1 ribbon-helix-helix domain-containing protein Antitoxin
CCGE532_RS21200 473976..474341 + 366 WP_120667679.1 type II toxin-antitoxin system VapC family toxin Toxin
CCGE532_RS21205 474464..474778 - 315 WP_120667681.1 hypothetical protein -
CCGE532_RS21210 475057..475740 - 684 WP_120667683.1 5-formyltetrahydrofolate cyclo-ligase -
CCGE532_RS21215 475856..476761 + 906 WP_120667685.1 LysR family transcriptional regulator -
CCGE532_RS21220 476815..477936 + 1122 WP_120667687.1 HPP family protein -
CCGE532_RS21225 478002..479132 - 1131 WP_120669294.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA 1..1460443 1460443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13907.37 Da        Isoelectric Point: 8.5623

>T112155 WP_120667679.1 NZ_CP032690:473976-474341 [Rhizobium sp. CCGE532]
MVKALFDTNILIDHLNAIPQARRELMRYEEKAISIITWMEVLAGAKPTVIVGTRAFLTGFIVIAVDDHIAERAVYLRQQH
RIKLPDAIIWATANVHSMLLVTRNTKDFPREMPDIRVPYEI

Download         Length: 366 bp

>T112155 NZ_CP032690:473976-474341 [Rhizobium sp. CCGE532]
ATGGTAAAAGCGCTTTTCGACACCAACATCTTGATCGACCATCTCAATGCCATACCGCAAGCGCGACGTGAGTTGATGCG
GTATGAGGAAAAGGCGATTAGCATCATCACCTGGATGGAGGTTCTCGCCGGTGCCAAACCGACGGTGATAGTCGGAACAC
GCGCTTTCCTAACAGGATTTATAGTCATCGCTGTCGATGATCACATAGCCGAGCGTGCGGTATATCTGCGACAGCAACAT
CGCATCAAACTGCCCGACGCCATCATCTGGGCAACGGCCAATGTTCATTCAATGTTGCTGGTTACGCGGAACACCAAGGA
TTTTCCGAGGGAAATGCCGGACATTCGAGTGCCTTACGAAATCTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8088.15 Da        Isoelectric Point: 4.5742

>AT112155 WP_120669292.1 NZ_CP032690:473767-473982 [Rhizobium sp. CCGE532]
MRTLVDIGDPEIKALDQLAQREKVSRAALIRKAIDDFLARNASISEVEAFGLWGDRKVDGLAYQEDIRNEW

Download         Length: 216 bp

>AT112155 NZ_CP032690:473767-473982 [Rhizobium sp. CCGE532]
ATGAGAACCTTGGTTGACATTGGCGACCCGGAGATCAAAGCGCTGGATCAGCTGGCGCAGCGAGAAAAAGTCTCGAGAGC
AGCACTCATTCGCAAGGCGATTGATGATTTTCTGGCTCGCAACGCAAGTATTAGCGAAGTCGAGGCGTTTGGGCTCTGGG
GTGATCGAAAGGTCGATGGTTTGGCGTATCAGGAAGACATTCGCAACGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References