Detailed information of TA system TA112151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2405881..2406539
• Replicon: chromosome
• Accession: NZ_CP032689
• Organism: Rhizobium sp. CCGE532

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCGE532_RS11850
• Protein ID: WP_120664436.1
• Coordinates: 2406117..2406539 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCGE532_RS11845
• Protein ID: WP_120664435.1
• Coordinates: 2405881..2406120 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE532_RS11825	2402390..2402734	-	345	WP_120664431.1	RidA family protein	-
CCGE532_RS11830	2402822..2403160	-	339	WP_120664432.1	TIGR01244 family phosphatase	-
CCGE532_RS11835	2403232..2404071	-	840	WP_120664433.1	DUF4432 family protein	-
CCGE532_RS11840	2404288..2405742	+	1455	WP_120664434.1	L,D-transpeptidase	-
CCGE532_RS11845	2405881..2406120	+	240	WP_120664435.1	Arc family DNA-binding protein	Antitoxin
CCGE532_RS11850	2406117..2406539	+	423	WP_120664436.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCGE532_RS11855	2406597..2407745	-	1149	WP_120664437.1	iron-containing alcohol dehydrogenase	-
CCGE532_RS11860	2407742..2409127	-	1386	WP_120664438.1	aldehyde dehydrogenase family protein	-
CCGE532_RS11865	2409249..2410616	-	1368	WP_120664439.1	glutamine synthetase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-41)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14842.11 Da
• Isoelectric Point: 5.1037

>T112151 WP_120664436.1 NZ_CP032689:2406117-2406539 [Rhizobium sp. CCGE532]
MIILDTNVVSEPMKPTGNDQVLKWLDQQLAETLFLTSTSLAELLVGIEILPDGKRKMGLDGALTQLVSRLFASRILAFDK
AAAENYAPLISRARSAGYSIAVADGQIAAIAATHGFAVATRDTAPFVAAGISTINPWKEG

Nucleotide

• Length: 423 bp

>T112151 NZ_CP032689:2406117-2406539 [Rhizobium sp. CCGE532]
ATGATTATTCTCGACACAAATGTCGTCTCGGAACCGATGAAGCCCACGGGCAATGATCAAGTTTTGAAATGGCTGGACCA
GCAGCTGGCGGAAACGCTTTTCCTGACGAGCACAAGCCTCGCCGAACTGTTGGTCGGTATAGAGATTCTGCCGGATGGCA
AACGCAAGATGGGCCTTGATGGCGCGCTCACCCAACTTGTTTCACGGCTCTTTGCTTCACGCATTCTTGCCTTCGACAAG
GCCGCCGCAGAAAATTACGCACCTTTAATCAGCCGCGCGAGATCCGCAGGATACTCGATTGCCGTCGCGGATGGCCAAAT
CGCGGCAATTGCGGCAACACATGGTTTCGCCGTCGCCACGCGAGATACAGCTCCATTCGTGGCCGCAGGAATCTCGACAA
TCAATCCCTGGAAAGAAGGGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8941.06 Da
• Isoelectric Point: 5.1992

>AT112151 WP_120664435.1 NZ_CP032689:2405881-2406120 [Rhizobium sp. CCGE532]
MAAITVRNLPDETHRALKLRAEQHGRSTEAEIREILEEAVRPKDRLKIGSELAAFGRQFGGLDLDLERERTPTEPASFE

Nucleotide

• Length: 240 bp

>AT112151 NZ_CP032689:2405881-2406120 [Rhizobium sp. CCGE532]
ATGGCTGCCATTACCGTAAGAAATCTTCCCGATGAAACTCACCGAGCGCTGAAGCTGCGAGCCGAGCAACATGGACGCAG
CACCGAAGCTGAAATCCGTGAAATTCTGGAGGAAGCTGTGCGTCCAAAGGACCGGCTGAAGATCGGCTCGGAATTGGCAG
CATTTGGGCGCCAGTTTGGTGGTTTGGATCTCGATTTGGAGCGTGAGCGGACGCCGACAGAACCGGCTAGTTTCGAATGA