Detailed information of TA system TA112150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1055565..1056045
• Replicon: chromosome
• Accession: NZ_CP032689
• Organism: Rhizobium sp. CCGE532

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: CCGE532_RS05225
• Protein ID: WP_120663233.1
• Coordinates: 1055773..1056045 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: CCGE532_RS05220
• Protein ID: WP_120663232.1
• Coordinates: 1055565..1055789 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE532_RS05190	1050987..1052117	+	1131	WP_120663227.1	site-specific DNA-methyltransferase	-
CCGE532_RS05195	1052250..1052615	-	366	WP_120663228.1	nuclear transport factor 2 family protein	-
CCGE532_RS05200	1052620..1053234	-	615	WP_120663229.1	HAD family phosphatase	-
CCGE532_RS05205	1053252..1054346	-	1095	WP_120663230.1	A/G-specific adenine glycosylase	-
CCGE532_RS05210	1054457..1054909	+	453	WP_120663231.1	hypothetical protein	-
CCGE532_RS05215	1054986..1055486	+	501	WP_120666511.1	DUF721 domain-containing protein	-
CCGE532_RS05220	1055565..1055789	+	225	WP_120663232.1	TraY domain-containing protein	Antitoxin
CCGE532_RS05225	1055773..1056045	+	273	WP_120663233.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCGE532_RS05230	1056219..1056908	+	690	WP_120663234.1	DsbA family protein	-
CCGE532_RS05235	1057095..1060556	+	3462	WP_120663235.1	chromosome segregation protein SMC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10703.34 Da
• Isoelectric Point: 10.7346

>T112150 WP_120663233.1 NZ_CP032689:1055773-1056045 [Rhizobium sp. CCGE532]
MAWRIEFQRAAERELDKLGHEASRRILRFLHDRVAKLDDPRAIGEALKGSELGNFWKYRVGDYRVIAHIEDSTVRILIVR
IGNRREVYRK

Nucleotide

• Length: 273 bp

>T112150 NZ_CP032689:1055773-1056045 [Rhizobium sp. CCGE532]
ATGGCGTGGCGGATTGAGTTTCAACGAGCTGCCGAGCGCGAACTGGACAAACTTGGGCACGAGGCTTCCCGCCGGATTTT
GAGATTTCTCCATGACCGCGTTGCCAAGCTGGATGATCCGCGCGCCATCGGAGAAGCGTTGAAAGGCTCGGAGCTCGGCA
ATTTCTGGAAATACCGAGTGGGGGATTATCGAGTGATCGCGCACATCGAAGATAGTACCGTTCGGATACTGATTGTTCGA
ATTGGAAATAGGCGCGAAGTCTATCGAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8508.67 Da
• Isoelectric Point: 4.6909

>AT112150 WP_120663232.1 NZ_CP032689:1055565-1055789 [Rhizobium sp. CCGE532]
MLALRLPPEIEARLDELAKRTGRSKSFYAREAILEHLDDLEDIYLAEKRLEEFRRGENGSVSLAELMARYGVAD

Nucleotide

• Length: 225 bp

>AT112150 NZ_CP032689:1055565-1055789 [Rhizobium sp. CCGE532]
ATGTTGGCCCTAAGGCTTCCGCCGGAAATTGAAGCAAGACTCGATGAACTCGCCAAGCGTACCGGGCGCAGCAAAAGTTT
CTATGCGCGCGAAGCAATTCTTGAGCATCTTGATGATCTTGAGGATATCTATCTGGCGGAGAAACGTCTGGAAGAGTTTC
GCCGCGGCGAGAATGGCAGCGTTTCGCTGGCCGAACTGATGGCGCGGTATGGCGTGGCGGATTGA