Detailed information of TA system TA112147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 506309..506853
• Replicon: plasmid pRCCGE531b
• Accession: NZ_CP032687
• Organism: Rhizobium sp. CCGE531

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: N6V6D9
• Locus tag: CCGE531_RS32300
• Protein ID: WP_004119734.1
• Coordinates: 506530..506853 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CCGE531_RS32295
• Protein ID: WP_120671022.1
• Coordinates: 506309..506533 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE531_RS32270	501449..502360	+	912	WP_120671021.1	LysR family transcriptional regulator	-
CCGE531_RS32275	502531..503715	+	1185	WP_120670959.1	pyridoxal phosphate-dependent aminotransferase	-
CCGE531_RS32280	503690..504562	+	873	WP_120670960.1	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
CCGE531_RS32290	505005..505910	+	906	WP_120670962.1	hypothetical protein	-
CCGE531_RS32295	506309..506533	+	225	WP_120671022.1	antitoxin MazE family protein	Antitoxin
CCGE531_RS32300	506530..506853	+	324	WP_004119734.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CCGE531_RS32305	506850..507200	-	351	WP_120670963.1	hypothetical protein	-
CCGE531_RS32310	507268..508083	+	816	WP_120670964.1	calcium-binding protein	-
CCGE531_RS32320	508952..509206	+	255	WP_120670966.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CCGE531_RS32325	509206..509631	+	426	WP_120670967.1	type II toxin-antitoxin system VapC family toxin	-
CCGE531_RS32330	510048..511451	-	1404	WP_120670968.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..577596	577596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-71)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11356.28 Da
• Isoelectric Point: 10.7342

>T112147 WP_004119734.1 NZ_CP032687:506530-506853 [Rhizobium sp. CCGE531]
VRRGDIVTVAISGDFGKPRPALIIQADQFDATGTITVLLISSTLVDAPLIRPTIQPTVQSGLRKASQVMVDKAMSVKRDR
IGTTIGRLEDDALLAVTRSLAVFLGVA

Nucleotide

• Length: 324 bp

>T112147 NZ_CP032687:506530-506853 [Rhizobium sp. CCGE531]
GTGAGGCGCGGTGACATTGTGACCGTCGCAATCAGCGGTGATTTCGGCAAGCCGCGTCCAGCACTAATCATCCAGGCCGA
TCAGTTTGATGCAACTGGAACGATCACTGTCCTGCTGATTTCCAGCACACTGGTCGACGCGCCGCTCATTCGACCAACCA
TTCAGCCAACAGTACAAAGCGGATTGCGAAAAGCCTCGCAAGTGATGGTGGACAAAGCCATGTCCGTGAAACGGGATAGA
ATTGGTACAACGATCGGTCGCCTGGAAGATGATGCGCTCCTGGCGGTCACAAGATCGCTCGCCGTTTTTTTAGGTGTCGC
TTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8118.03 Da
• Isoelectric Point: 4.7377

>AT112147 WP_120671022.1 NZ_CP032687:506309-506533 [Rhizobium sp. CCGE531]
MASSVNERVQKRRETLRAAGLRPVQIWVPDTRRPGFAAECHRQSVQVALADAADRGLQDFLDAALGDLDDEGEA

Nucleotide

• Length: 225 bp

>AT112147 NZ_CP032687:506309-506533 [Rhizobium sp. CCGE531]
ATGGCAAGCTCGGTCAACGAACGAGTCCAAAAGCGCCGTGAGACGTTGCGCGCCGCTGGGCTGCGTCCTGTGCAAATATG
GGTTCCTGATACTCGTCGCCCTGGTTTTGCCGCCGAATGCCATCGTCAGTCCGTTCAGGTAGCCCTTGCCGATGCCGCCG
ACCGCGGTCTGCAAGACTTCCTTGACGCCGCTCTCGGCGACCTGGACGACGAGGGTGAAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A5F1S1T4