Detailed information of TA system TA112146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 175576..176159
• Replicon: plasmid pRCCGE531c
• Accession: NZ_CP032686
• Organism: Rhizobium sp. CCGE531

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCGE531_RS27080
• Protein ID: WP_120669685.1
• Coordinates: 175576..175962 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCGE531_RS27085
• Protein ID: WP_120669687.1
• Coordinates: 175959..176159 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE531_RS27080	175576..175962	-	387	WP_120669685.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCGE531_RS27085	175959..176159	-	201	WP_120669687.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CCGE531_RS27090	176319..178096	-	1778	Protein_144	ABC transporter ATP-binding protein	-
CCGE531_RS27095	178104..178501	-	398	Protein_145	glycosyltransferase family 2 protein	-
CCGE531_RS27100	178503..179212	-	710	Protein_146	glycosyltransferase family 2 protein	-
CCGE531_RS27105	179494..179745	+	252	WP_120669689.1	transcriptional regulator	-
CCGE531_RS27110	179745..180107	+	363	WP_120669690.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..756635	756635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14118.24 Da
• Isoelectric Point: 6.7690

>T112146 WP_120669685.1 NZ_CP032686:c175962-175576 [Rhizobium sp. CCGE531]
VILADTSIWIDHFRSADTELRRIIEDDRLLCHPVVIGELALGSLRDRGDVIAFLSAQRGAVVATHDEVMIMIDRHGIFSM
GIGYTDAHLLASVLLDHKASLWTRDKRLRAAADKAGASLHIPVNAPHH

Nucleotide

• Length: 387 bp

>T112146 NZ_CP032686:c175962-175576 [Rhizobium sp. CCGE531]
GTGATACTTGCAGACACCTCGATCTGGATTGATCATTTCCGATCTGCCGATACAGAGCTGCGCAGGATTATCGAGGATGA
TCGTCTGCTCTGCCATCCGGTTGTGATTGGCGAGCTGGCGCTTGGCAGCCTCCGCGATCGCGGTGACGTGATAGCTTTCC
TATCGGCCCAGCGCGGTGCGGTCGTCGCAACGCATGACGAAGTCATGATAATGATCGATCGACATGGTATTTTCAGCATG
GGCATTGGCTACACGGATGCTCACTTGCTGGCGTCTGTTCTTCTCGACCACAAAGCGTCCTTATGGACCAGAGACAAGCG
CCTGCGGGCAGCGGCCGACAAGGCAGGAGCCTCACTGCATATACCGGTCAACGCGCCTCATCACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7090.24 Da
• Isoelectric Point: 11.1107

>AT112146 WP_120669687.1 NZ_CP032686:c176159-175959 [Rhizobium sp. CCGE531]
MRSTINLDDALLDRAKSLTGIKETAALVRQALETLVRVESGKRLIALGGTMPEAEAAPRRRSTAAK

Nucleotide

• Length: 201 bp

>AT112146 NZ_CP032686:c176159-175959 [Rhizobium sp. CCGE531]
ATGCGATCTACTATCAATCTCGACGATGCACTCTTGGATAGGGCGAAATCCTTGACTGGGATCAAGGAAACTGCGGCGCT
CGTTCGCCAGGCATTGGAGACGCTTGTTCGCGTAGAGTCCGGAAAACGCCTCATCGCGCTTGGAGGAACCATGCCTGAGG
CAGAGGCAGCTCCGCGCCGCCGGAGCACAGCGGCTAAGTGA