Detailed information of TA system TA112140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2550234..2550892
• Replicon: chromosome
• Accession: NZ_CP032684
• Organism: Rhizobium sp. CCGE531

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCGE531_RS12420
• Protein ID: WP_120664436.1
• Coordinates: 2550470..2550892 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CCGE531_RS12415
• Protein ID: WP_120664435.1
• Coordinates: 2550234..2550473 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE531_RS12395	2546743..2547087	-	345	WP_120664431.1	RidA family protein	-
CCGE531_RS12400	2547175..2547513	-	339	WP_120664432.1	TIGR01244 family phosphatase	-
CCGE531_RS12405	2547585..2548424	-	840	WP_120664433.1	DUF4432 family protein	-
CCGE531_RS12410	2548641..2550095	+	1455	WP_120664434.1	L,D-transpeptidase	-
CCGE531_RS12415	2550234..2550473	+	240	WP_120664435.1	Arc family DNA-binding protein	Antitoxin
CCGE531_RS12420	2550470..2550892	+	423	WP_120664436.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCGE531_RS12425	2551040..2552326	+	1287	Protein_2425	IS701 family transposase	-
CCGE531_RS12430	2552475..2553623	-	1149	WP_120664437.1	iron-containing alcohol dehydrogenase	-
CCGE531_RS12435	2553620..2555005	-	1386	WP_120664438.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-41)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14842.11 Da
• Isoelectric Point: 5.1037

>T112140 WP_120664436.1 NZ_CP032684:2550470-2550892 [Rhizobium sp. CCGE531]
MIILDTNVVSEPMKPTGNDQVLKWLDQQLAETLFLTSTSLAELLVGIEILPDGKRKMGLDGALTQLVSRLFASRILAFDK
AAAENYAPLISRARSAGYSIAVADGQIAAIAATHGFAVATRDTAPFVAAGISTINPWKEG

Nucleotide

• Length: 423 bp

>T112140 NZ_CP032684:2550470-2550892 [Rhizobium sp. CCGE531]
ATGATTATTCTCGACACAAATGTCGTCTCGGAACCGATGAAGCCCACGGGCAATGATCAAGTTTTGAAATGGCTGGACCA
GCAGCTGGCGGAAACGCTTTTCCTGACGAGCACAAGCCTCGCCGAACTGTTGGTCGGTATAGAGATTCTGCCGGATGGCA
AACGCAAGATGGGCCTTGATGGCGCGCTCACCCAACTTGTTTCACGGCTCTTTGCTTCACGCATTCTTGCCTTCGACAAG
GCCGCCGCAGAAAATTACGCACCTTTAATCAGCCGCGCGAGATCCGCAGGATACTCGATTGCCGTCGCGGATGGCCAAAT
CGCGGCAATTGCGGCAACACATGGTTTCGCCGTCGCCACGCGAGATACAGCTCCATTCGTGGCCGCAGGAATCTCGACAA
TCAATCCCTGGAAAGAAGGGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8941.06 Da
• Isoelectric Point: 5.1992

>AT112140 WP_120664435.1 NZ_CP032684:2550234-2550473 [Rhizobium sp. CCGE531]
MAAITVRNLPDETHRALKLRAEQHGRSTEAEIREILEEAVRPKDRLKIGSELAAFGRQFGGLDLDLERERTPTEPASFE

Nucleotide

• Length: 240 bp

>AT112140 NZ_CP032684:2550234-2550473 [Rhizobium sp. CCGE531]
ATGGCTGCCATTACCGTAAGAAATCTTCCCGATGAAACTCACCGAGCGCTGAAGCTGCGAGCCGAGCAACATGGACGCAG
CACCGAAGCTGAAATCCGTGAAATTCTGGAGGAAGCTGTGCGTCCAAAGGACCGGCTGAAGATCGGCTCGGAATTGGCAG
CATTTGGGCGCCAGTTTGGTGGTTTGGATCTCGATTTGGAGCGTGAGCGGACGCCGACAGAACCGGCTAGTTTCGAATGA