Detailed information of TA system TA112139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1058071..1058551
• Replicon: chromosome
• Accession: NZ_CP032684
• Organism: Rhizobium sp. CCGE531

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: CCGE531_RS05225
• Protein ID: WP_120663233.1
• Coordinates: 1058279..1058551 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: CCGE531_RS05220
• Protein ID: WP_120663232.1
• Coordinates: 1058071..1058295 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCGE531_RS05190	1053493..1054623	+	1131	WP_120663227.1	site-specific DNA-methyltransferase	-
CCGE531_RS05195	1054756..1055121	-	366	WP_120663228.1	nuclear transport factor 2 family protein	-
CCGE531_RS05200	1055126..1055740	-	615	WP_120663229.1	HAD family phosphatase	-
CCGE531_RS05205	1055758..1056852	-	1095	WP_120663230.1	A/G-specific adenine glycosylase	-
CCGE531_RS05210	1056963..1057415	+	453	WP_120663231.1	hypothetical protein	-
CCGE531_RS05215	1057492..1057992	+	501	WP_120666511.1	DUF721 domain-containing protein	-
CCGE531_RS05220	1058071..1058295	+	225	WP_120663232.1	TraY domain-containing protein	Antitoxin
CCGE531_RS05225	1058279..1058551	+	273	WP_120663233.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCGE531_RS05230	1058725..1059414	+	690	WP_120663234.1	DsbA family protein	-
CCGE531_RS05235	1059601..1063062	+	3462	WP_120663235.1	chromosome segregation protein SMC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10703.34 Da
• Isoelectric Point: 10.7346

>T112139 WP_120663233.1 NZ_CP032684:1058279-1058551 [Rhizobium sp. CCGE531]
MAWRIEFQRAAERELDKLGHEASRRILRFLHDRVAKLDDPRAIGEALKGSELGNFWKYRVGDYRVIAHIEDSTVRILIVR
IGNRREVYRK

Nucleotide

• Length: 273 bp

>T112139 NZ_CP032684:1058279-1058551 [Rhizobium sp. CCGE531]
ATGGCGTGGCGGATTGAGTTTCAACGAGCTGCCGAGCGCGAACTGGACAAACTTGGGCACGAGGCTTCCCGCCGGATTTT
GAGATTTCTCCATGACCGCGTTGCCAAGCTGGATGATCCGCGCGCCATCGGAGAAGCGTTGAAAGGCTCGGAGCTCGGCA
ATTTCTGGAAATACCGAGTGGGGGATTATCGAGTGATCGCGCACATCGAAGATAGTACCGTTCGGATACTGATTGTTCGA
ATTGGAAATAGGCGCGAAGTCTATCGAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8508.67 Da
• Isoelectric Point: 4.6909

>AT112139 WP_120663232.1 NZ_CP032684:1058071-1058295 [Rhizobium sp. CCGE531]
MLALRLPPEIEARLDELAKRTGRSKSFYAREAILEHLDDLEDIYLAEKRLEEFRRGENGSVSLAELMARYGVAD

Nucleotide

• Length: 225 bp

>AT112139 NZ_CP032684:1058071-1058295 [Rhizobium sp. CCGE531]
ATGTTGGCCCTAAGGCTTCCGCCGGAAATTGAAGCAAGACTCGATGAACTCGCCAAGCGTACCGGGCGCAGCAAAAGTTT
CTATGCGCGCGAAGCAATTCTTGAGCATCTTGATGATCTTGAGGATATCTATCTGGCGGAGAAACGTCTGGAAGAGTTTC
GCCGCGGCGAGAATGGCAGCGTTTCGCTGGCCGAACTGATGGCGCGGTATGGCGTGGCGGATTGA