Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1398938..1399462 Replicon chromosome
Accession NZ_CP032682
Organism Staphylococcus pseudintermedius strain G3C4

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag D8L98_RS06485 Protein ID WP_014613379.1
Coordinates 1399109..1399462 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag D8L98_RS06480 Protein ID WP_014613378.1
Coordinates 1398938..1399108 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8L98_RS06455 1394827..1395300 + 474 WP_037542551.1 PH domain-containing protein -
D8L98_RS06460 1395293..1396822 + 1530 WP_037542554.1 PH domain-containing protein -
D8L98_RS06465 1396806..1397324 + 519 WP_014613375.1 PH domain-containing protein -
D8L98_RS06470 1397321..1397671 + 351 WP_037542556.1 holo-ACP synthase -
D8L98_RS06475 1397705..1398853 + 1149 WP_014613377.1 alanine racemase -
D8L98_RS06480 1398938..1399108 + 171 WP_014613378.1 hypothetical protein Antitoxin
D8L98_RS06485 1399109..1399462 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D8L98_RS06490 1399520..1400524 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
D8L98_RS06495 1400603..1400929 + 327 WP_014613381.1 anti-sigma factor antagonist -
D8L98_RS06500 1400929..1401408 + 480 WP_031253870.1 anti-sigma B factor RsbW -
D8L98_RS06505 1401383..1402153 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T112136 WP_014613379.1 NZ_CP032682:1399109-1399462 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T112136 NZ_CP032682:1399109-1399462 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT112136 WP_014613378.1 NZ_CP032682:1398938-1399108 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT112136 NZ_CP032682:1398938-1399108 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References