Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4438338..4438933 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag D7U33_RS21370 Protein ID WP_000239577.1
Coordinates 4438583..4438933 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag D7U33_RS21365 Protein ID WP_001223208.1
Coordinates 4438338..4438589 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS21355 4434003..4437782 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
D7U33_RS21360 4437785..4438126 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
D7U33_RS21365 4438338..4438589 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
D7U33_RS21370 4438583..4438933 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
D7U33_RS21375 4439013..4439543 - 531 WP_000055075.1 inorganic diphosphatase -
D7U33_RS21380 4439853..4440809 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
D7U33_RS21385 4440949..4442451 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
D7U33_RS21390 4442465..4443487 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T112128 WP_000239577.1 NZ_CP032679:4438583-4438933 [Escherichia coli str. K-12 substr. MG1655]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T112128 NZ_CP032679:4438583-4438933 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT112128 WP_001223208.1 NZ_CP032679:4438338-4438589 [Escherichia coli str. K-12 substr. MG1655]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT112128 NZ_CP032679:4438338-4438589 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References