Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2777118..2777785 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag D7U33_RS13520 Protein ID WP_001094400.1
Coordinates 2777456..2777785 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag D7U33_RS13515 Protein ID WP_000072690.1
Coordinates 2777118..2777435 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS13485 2772170..2773179 - 1010 Protein_2635 arsenic transporter -
D7U33_RS13490 2773321..2775024 + 1704 WP_000896263.1 hypothetical protein -
D7U33_RS13495 2775596..2775819 + 224 Protein_2637 DUF905 family protein -
D7U33_RS13500 2775922..2776380 + 459 WP_000211841.1 antirestriction protein -
D7U33_RS13505 2776389..2776871 + 483 WP_001407480.1 RadC family protein -
D7U33_RS13510 2776880..2777080 + 201 WP_001395452.1 DUF987 domain-containing protein -
D7U33_RS13515 2777118..2777435 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D7U33_RS13520 2777456..2777785 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
D7U33_RS13525 2778149..2782729 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T112119 WP_001094400.1 NZ_CP032679:2777456-2777785 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T112119 NZ_CP032679:2777456-2777785 [Escherichia coli str. K-12 substr. MG1655]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT112119 WP_000072690.1 NZ_CP032679:2777118-2777435 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT112119 NZ_CP032679:2777118-2777435 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References