Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2077117..2077948 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag D7U33_RS10240 Protein ID WP_000854814.1
Coordinates 2077574..2077948 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag D7U33_RS10235 Protein ID WP_001285584.1
Coordinates 2077117..2077485 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS10220 2074784..2076316 + 1533 WP_001350525.1 hypothetical protein -
D7U33_RS10225 2076313..2076759 + 447 WP_000187523.1 RadC family protein -
D7U33_RS10230 2076822..2077043 + 222 WP_000692323.1 DUF987 domain-containing protein -
D7U33_RS10235 2077117..2077485 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
D7U33_RS10240 2077574..2077948 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
D7U33_RS10245 2077945..2078139 + 195 WP_000988600.1 hypothetical protein -
D7U33_RS10250 2078152..2078265 + 114 WP_001161660.1 DUF957 domain-containing protein -
D7U33_RS10255 2078554..2078682 - 129 Protein_2003 transposase domain-containing protein -
D7U33_RS10260 2078754..2078936 + 183 WP_001016348.1 hypothetical protein -
D7U33_RS10265 2079037..2079366 - 330 WP_000450409.1 DUF496 family protein -
D7U33_RS10270 2079538..2080596 - 1059 WP_001200891.1 FUSC family protein -
D7U33_RS10275 2080794..2081267 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
D7U33_RS10280 2081386..2082552 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T112116 WP_000854814.1 NZ_CP032679:2077574-2077948 [Escherichia coli str. K-12 substr. MG1655]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T112116 NZ_CP032679:2077574-2077948 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT112116 WP_001285584.1 NZ_CP032679:2077117-2077485 [Escherichia coli str. K-12 substr. MG1655]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT112116 NZ_CP032679:2077117-2077485 [Escherichia coli str. K-12 substr. MG1655]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References