Detailed information of TA system TA112105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1509291..1509929
• Replicon: chromosome
• Accession: NZ_CP032679
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D7U33_RS07355
• Protein ID: WP_000813794.1
• Coordinates: 1509291..1509467 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7U33_RS07360
• Protein ID: WP_001270286.1
• Coordinates: 1509513..1509929 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7U33_RS07335	1504910..1506124	-	1215	WP_001326689.1	BenE family transporter YdcO	-
D7U33_RS07340	1506177..1506713	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
D7U33_RS07345	1506786..1508747	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D7U33_RS07350	1508839..1509069	-	231	WP_000494244.1	YncJ family protein	-
D7U33_RS07355	1509291..1509467	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D7U33_RS07360	1509513..1509929	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D7U33_RS07365	1510008..1511414	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
D7U33_RS07370	1511659..1512804	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
D7U33_RS07375	1512822..1513835	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
D7U33_RS07380	1513836..1514777	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T112105 WP_000813794.1 NZ_CP032679:1509291-1509467 [Escherichia coli str. K-12 substr. MG1655]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T112105 NZ_CP032679:1509291-1509467 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT112105 WP_001270286.1 NZ_CP032679:1509513-1509929 [Escherichia coli str. K-12 substr. MG1655]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT112105 NZ_CP032679:1509513-1509929 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB