Detailed information of TA system TA112093

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263331..264010
• Replicon: chromosome
• Accession: NZ_CP032679
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: D7U33_RS01250
• Protein ID: WP_000854672.1
• Coordinates: 263331..263672 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: D7U33_RS01255
• Protein ID: WP_000070395.1
• Coordinates: 263693..264010 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7U33_RS01225	258621..259009	+	389	Protein_237	sigma factor-binding protein Crl	-
D7U33_RS01230	259048..260103	-	1056	WP_000749863.1	phosphoporin PhoE	-
D7U33_RS01235	260391..261494	+	1104	WP_001285288.1	glutamate 5-kinase	-
D7U33_RS01240	261506..262759	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
D7U33_RS01250	263331..263672	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
D7U33_RS01255	263693..264010	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
D7U33_RS01260	264029..264250	-	222	WP_000691994.1	DUF987 domain-containing protein	-
D7U33_RS01265	264259..264735	-	477	WP_000811693.1	RadC family protein	-
D7U33_RS01270	264751..265209	-	459	WP_000211838.1	antirestriction protein	-
D7U33_RS01275	265307..265546	-	240	WP_000194654.1	DUF905 domain-containing protein	-
D7U33_RS01280	265623..266090	-	468	WP_001547765.1	hypothetical protein	-
D7U33_RS01285	266113..266556	-	444	WP_000824223.1	hypothetical protein	-
D7U33_RS01290	266556..266783	-	228	WP_001548158.1	hypothetical protein	-
D7U33_RS01295	266779..266970	-	192	Protein_250	DeoR family transcriptional regulator	-
D7U33_RS01300	267187..268008	-	822	WP_000197389.1	DUF945 domain-containing protein	-
D7U33_RS01305	268100..268963	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T112093 WP_000854672.1 NZ_CP032679:c263672-263331 [Escherichia coli str. K-12 substr. MG1655]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T112093 NZ_CP032679:c263672-263331 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT112093 WP_000070395.1 NZ_CP032679:c264010-263693 [Escherichia coli str. K-12 substr. MG1655]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT112093 NZ_CP032679:c264010-263693 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684