Detailed information of TA system TA112090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 278343..278983
• Replicon: plasmid pBASL58
• Accession: NZ_CP032678
• Organism: Pseudomonas sp. Leaf58

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DV532_RS26700
• Protein ID: WP_056799328.1
• Coordinates: 278573..278983 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DV532_RS26695
• Protein ID: WP_056799331.1
• Coordinates: 278343..278573 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV532_RS26660	273686..274357	-	672	WP_120715439.1	hypothetical protein	-
DV532_RS26665	274485..274796	+	312	WP_056799349.1	hypothetical protein	-
DV532_RS26670	274875..275492	-	618	WP_056799344.1	hypothetical protein	-
DV532_RS26675	275789..276553	+	765	WP_056799343.1	hypothetical protein	-
DV532_RS26680	276621..277232	+	612	WP_162241131.1	hypothetical protein	-
DV532_RS26685	277262..277519	+	258	WP_056799337.1	hypothetical protein	-
DV532_RS26690	277828..278187	+	360	WP_056799334.1	hypothetical protein	-
DV532_RS26695	278343..278573	+	231	WP_056799331.1	antitoxin	Antitoxin
DV532_RS26700	278573..278983	+	411	WP_056799328.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DV532_RS26705	279001..279252	+	252	WP_056799325.1	hypothetical protein	-
DV532_RS26710	279341..279580	+	240	WP_056799319.1	hypothetical protein	-
DV532_RS26715	279592..279888	+	297	WP_156675953.1	hypothetical protein	-
DV532_RS26725	280463..281128	+	666	WP_056799313.1	hypothetical protein	-
DV532_RS26730	281353..281637	+	285	WP_056799310.1	hypothetical protein	-
DV532_RS30505	281643..281870	+	228	WP_156675951.1	hypothetical protein	-
DV532_RS26735	282033..282278	-	246	Protein_315	transposase	-
DV532_RS26740	282558..283211	+	654	WP_056799307.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tufA	1..904163	904163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (7-34)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15382.75 Da
• Isoelectric Point: 7.2927

>T112090 WP_056799328.1 NZ_CP032678:278573-278983 [Pseudomonas sp. Leaf58]
MLKFMLDTNICIFTIKNRPQEVREVFKRHHGQMCISTVTLMELIYGAEKSSNPERNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRCEFDRVPGLRIEDWVAPIA

Nucleotide

• Length: 411 bp

>T112090 NZ_CP032678:278573-278983 [Pseudomonas sp. Leaf58]
ATGCTCAAATTCATGCTCGACACCAACATTTGCATCTTCACCATCAAGAACCGTCCCCAAGAGGTGCGTGAGGTTTTCAA
GCGGCATCACGGCCAGATGTGCATCAGCACCGTGACGCTAATGGAGCTTATCTACGGTGCGGAGAAGTCCTCGAACCCCG
AGCGCAATCTTGCGGACGTAGAAGGCTTTGCGGCTCGACTGGAGGTGCTCAAGTATGATCAAGAAGCTGCCGCCCACACT
GGTCAGCTTCGTGCTGAACTGGCCCGTCTTGGTAAACAAATTGGCCCCTATGACCAGATGATCGCTGGGCATGCCCGTTC
TCAGGGGCTGATTGTTGTCACTAACAACCGCTGCGAGTTCGACCGCGTGCCCGGTTTAAGAATTGAGGATTGGGTCGCCC
CGATCGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8476.40 Da
• Isoelectric Point: 4.2821

>AT112090 WP_056799331.1 NZ_CP032678:278343-278573 [Pseudomonas sp. Leaf58]
VEQGAVFQSNRSQAVRLPKAVALPDDVKRVDVVALGRARIITPAGESWDSWFDSEGVTVDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT112090 NZ_CP032678:278343-278573 [Pseudomonas sp. Leaf58]
GTGGAACAAGGCGCCGTTTTTCAGAGCAATCGCAGCCAGGCGGTGAGGCTACCCAAGGCTGTTGCCCTTCCTGATGACGT
GAAGCGTGTCGATGTGGTGGCTCTTGGTCGAGCCCGGATCATTACCCCTGCTGGCGAGTCTTGGGATAGCTGGTTCGACA
GCGAAGGCGTAACTGTTGACTTCATGGCGGAGCGCGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA