Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1805429..1806260 Replicon chromosome
Accession NZ_CP032667
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag D8B36_RS08775 Protein ID WP_000854814.1
Coordinates 1805429..1805803 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag D8B36_RS08780 Protein ID WP_001285584.1
Coordinates 1805892..1806260 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B36_RS08735 1800825..1801991 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
D8B36_RS08740 1802110..1802583 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
D8B36_RS08745 1802781..1803839 + 1059 WP_001200891.1 FUSC family protein -
D8B36_RS08750 1804011..1804340 + 330 WP_000450409.1 DUF496 family protein -
D8B36_RS08755 1804441..1804623 - 183 WP_001016348.1 hypothetical protein -
D8B36_RS08760 1804695..1804823 + 129 Protein_1675 transposase domain-containing protein -
D8B36_RS08765 1805112..1805225 - 114 WP_001161660.1 DUF957 domain-containing protein -
D8B36_RS08770 1805238..1805432 - 195 WP_000988600.1 hypothetical protein -
D8B36_RS08775 1805429..1805803 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
D8B36_RS08780 1805892..1806260 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
D8B36_RS08785 1806334..1806555 - 222 WP_000692323.1 DUF987 domain-containing protein -
D8B36_RS08790 1806618..1807064 - 447 WP_000187523.1 RadC family protein -
D8B36_RS08795 1807061..1808593 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T112066 WP_000854814.1 NZ_CP032667:c1805803-1805429 [Escherichia coli str. K-12 substr. MG1655]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T112066 NZ_CP032667:c1805803-1805429 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT112066 WP_001285584.1 NZ_CP032667:c1806260-1805892 [Escherichia coli str. K-12 substr. MG1655]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT112066 NZ_CP032667:c1806260-1805892 [Escherichia coli str. K-12 substr. MG1655]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References