Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 10593..11194 Replicon plasmid unnamed2
Accession NZ_CP032661
Organism Lactiplantibacillus pentosus strain ZFM94

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag CFI14_RS16980 Protein ID WP_001748110.1
Coordinates 10850..11194 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag CFI14_RS16975 Protein ID WP_001748109.1
Coordinates 10593..10856 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI14_RS16955 6028..6285 + 258 WP_080343736.1 head-tail connector protein -
CFI14_RS16960 6979..7467 + 489 WP_046811101.1 DUF536 domain-containing protein -
CFI14_RS16965 9352..9546 - 195 WP_046811110.1 hypothetical protein -
CFI14_RS16970 9920..10507 - 588 WP_046811109.1 site-specific integrase -
CFI14_RS16975 10593..10856 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CFI14_RS16980 10850..11194 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CFI14_RS16985 11764..12444 - 681 WP_046811108.1 ABC transporter ATP-binding protein -
CFI14_RS16990 12449..13501 - 1053 WP_120769740.1 ABC transporter permease -
CFI14_RS16995 13627..13848 + 222 WP_046811106.1 transposase -
CFI14_RS17000 14179..14457 + 279 WP_003625288.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
CFI14_RS17005 14447..14749 + 303 WP_005917927.1 type II toxin-antitoxin system YafQ family toxin -
CFI14_RS17010 14872..15282 - 411 WP_046811105.1 hypothetical protein -
CFI14_RS17015 15398..15805 - 408 WP_046811104.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..27706 27706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T112044 WP_001748110.1 NZ_CP032661:10850-11194 [Lactiplantibacillus pentosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T112044 NZ_CP032661:10850-11194 [Lactiplantibacillus pentosus]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT112044 WP_001748109.1 NZ_CP032661:10593-10856 [Lactiplantibacillus pentosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT112044 NZ_CP032661:10593-10856 [Lactiplantibacillus pentosus]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References