Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 6021..6622 Replicon plasmid unnamed3
Accession NZ_CP032657
Organism Lactiplantibacillus pentosus strain ZFM222

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag CFK27_RS17120 Protein ID WP_001748110.1
Coordinates 6021..6365 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag CFK27_RS17125 Protein ID WP_001748109.1
Coordinates 6359..6622 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFK27_RS17085 1410..1817 + 408 WP_046811104.1 hypothetical protein -
CFK27_RS17090 1933..2343 + 411 WP_046811105.1 hypothetical protein -
CFK27_RS17095 2466..2768 - 303 WP_005917927.1 type II toxin-antitoxin system YafQ family toxin -
CFK27_RS17100 2758..3036 - 279 WP_003625288.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
CFK27_RS17105 3367..3588 - 222 WP_046811106.1 transposase -
CFK27_RS17110 3714..4766 + 1053 WP_120769740.1 ABC transporter permease -
CFK27_RS17115 4771..5451 + 681 WP_120769741.1 ABC transporter ATP-binding protein -
CFK27_RS17120 6021..6365 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CFK27_RS17125 6359..6622 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CFK27_RS17130 6708..7295 + 588 WP_120769742.1 site-specific integrase -
CFK27_RS17135 7669..7863 + 195 WP_046811110.1 hypothetical protein -
CFK27_RS17140 9748..10236 - 489 WP_046811101.1 DUF536 domain-containing protein -
CFK27_RS17145 10930..11187 - 258 WP_080343736.1 head-tail connector protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..13846 13846


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T112043 WP_001748110.1 NZ_CP032657:c6365-6021 [Lactiplantibacillus pentosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T112043 NZ_CP032657:c6365-6021 [Lactiplantibacillus pentosus]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT112043 WP_001748109.1 NZ_CP032657:c6622-6359 [Lactiplantibacillus pentosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT112043 NZ_CP032657:c6622-6359 [Lactiplantibacillus pentosus]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References