112040

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 2650..3264 Replicon plasmid pM228
Accession NZ_CP032632
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name PfiT Uniprot ID A0A2V0R8Y7
Locus tag CN228_RS32405 Protein ID WP_017685123.1
Coordinates 2914..3264 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID S6SUK0
Locus tag CN228_RS32400 Protein ID WP_017685122.1
Coordinates 2650..2898 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS32385 80..1149 + 1070 Protein_0 IS630 family transposase -
CN228_RS32395 1347..2309 - 963 WP_017685121.1 tyrosine-type recombinase/integrase -
CN228_RS32400 2650..2898 + 249 WP_017685122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CN228_RS32405 2914..3264 + 351 WP_017685123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CN228_RS32410 3396..3980 - 585 Protein_4 helix-turn-helix transcriptional regulator -
CN228_RS32420 5687..5992 + 306 WP_017706770.1 type IV secretion system protein -
CN228_RS32425 5982..6791 + 810 WP_017684942.1 P-type conjugative transfer protein VirB9 -
CN228_RS32430 6778..8148 + 1371 WP_017684943.1 TrbI/VirB10 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..72748 72748
- inside IScluster/Tn - - 416..5530 5114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-101)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13012.89 Da        Isoelectric Point: 4.1953

>T112040 WP_017685123.1 NZ_CP032632:2914-3264 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T112040 NZ_CP032632:2914-3264 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9389.72 Da        Isoelectric Point: 9.5019

>AT112040 WP_017685122.1 NZ_CP032632:2650-2898 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT112040 NZ_CP032632:2650-2898 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2V0R8Y7


Antitoxin

Source ID Structure
AlphaFold DB A0A2V0R8I6

References