Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | PfiT-PfiA/ParE-Phd |
Location | 2650..3264 | Replicon | plasmid pM228 |
Accession | NZ_CP032632 | ||
Organism | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
Toxin (Protein)
Gene name | PfiT | Uniprot ID | A0A2V0R8Y7 |
Locus tag | CN228_RS32405 | Protein ID | WP_017685123.1 |
Coordinates | 2914..3264 (+) | Length | 117 a.a. |
Antitoxin (Protein)
Gene name | PfiA | Uniprot ID | S6SUK0 |
Locus tag | CN228_RS32400 | Protein ID | WP_017685122.1 |
Coordinates | 2650..2898 (+) | Length | 83 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
CN228_RS32385 | 80..1149 | + | 1070 | Protein_0 | IS630 family transposase | - |
CN228_RS32395 | 1347..2309 | - | 963 | WP_017685121.1 | tyrosine-type recombinase/integrase | - |
CN228_RS32400 | 2650..2898 | + | 249 | WP_017685122.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
CN228_RS32405 | 2914..3264 | + | 351 | WP_017685123.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
CN228_RS32410 | 3396..3980 | - | 585 | Protein_4 | helix-turn-helix transcriptional regulator | - |
CN228_RS32420 | 5687..5992 | + | 306 | WP_017706770.1 | type IV secretion system protein | - |
CN228_RS32425 | 5982..6791 | + | 810 | WP_017684942.1 | P-type conjugative transfer protein VirB9 | - |
CN228_RS32430 | 6778..8148 | + | 1371 | WP_017684943.1 | TrbI/VirB10 family protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..72748 | 72748 | |
- | inside | IScluster/Tn | - | - | 416..5530 | 5114 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13012.89 Da Isoelectric Point: 4.1953
>T112040 WP_017685123.1 NZ_CP032632:2914-3264 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI
Download Length: 351 bp
>T112040 NZ_CP032632:2914-3264 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG
Antitoxin
Download Length: 83 a.a. Molecular weight: 9389.72 Da Isoelectric Point: 9.5019
>AT112040 WP_017685122.1 NZ_CP032632:2650-2898 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK
Download Length: 249 bp
>AT112040 NZ_CP032632:2650-2898 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2V0R8Y7 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2V0R8I6 |