Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5361491..5362032 Replicon chromosome
Accession NZ_CP032631
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name relE Uniprot ID A0A0K8LVV5
Locus tag CN228_RS26015 Protein ID WP_017683357.1
Coordinates 5361757..5362032 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0K8LU58
Locus tag CN228_RS26010 Protein ID WP_003382249.1
Coordinates 5361491..5361757 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS25995 5356774..5357037 + 264 WP_017683360.1 hypothetical protein -
CN228_RS26000 5357165..5359045 - 1881 WP_003382247.1 methyl-accepting chemotaxis protein -
CN228_RS26005 5359404..5361326 + 1923 WP_017683359.1 methyl-accepting chemotaxis protein -
CN228_RS26010 5361491..5361757 + 267 WP_003382249.1 ribbon-helix-helix domain-containing protein Antitoxin
CN228_RS26015 5361757..5362032 + 276 WP_017683357.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CN228_RS26030 5362604..5363872 + 1269 WP_007244158.1 OprD family porin -
CN228_RS26035 5364051..5364875 + 825 WP_003380380.1 NAD(P)-dependent oxidoreductase -
CN228_RS26040 5364935..5365819 + 885 WP_003380378.1 SMP-30/gluconolactonase/LRE family protein -
CN228_RS26045 5365876..5366907 + 1032 WP_020312848.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5284945..5364875 79930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(8-45)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10770.41 Da        Isoelectric Point: 7.0123

>T112037 WP_017683357.1 NZ_CP032631:5361757-5362032 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MELMWTSKTLSDVTRLYDFLAVANQPAAAQTAQKLTAAPIMLLSNPRIGERLEEFEPRDVRRIQIGRYEMRYEIVDSTIY
LLRLWHTREDR

Download         Length: 276 bp

>T112037 NZ_CP032631:5361757-5362032 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGGAGCTGATGTGGACAAGCAAAACGCTATCTGACGTCACCCGCCTGTATGATTTTTTAGCGGTAGCAAACCAGCCCGC
TGCCGCCCAGACCGCACAGAAGCTTACAGCGGCGCCGATCATGCTCCTTTCCAACCCGCGGATTGGTGAGCGTCTGGAGG
AGTTTGAGCCCCGGGATGTGCGCAGAATTCAGATAGGCCGCTATGAAATGCGCTACGAAATTGTGGACTCAACAATTTAT
CTGTTGCGGCTGTGGCATACACGCGAGGATCGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9934.24 Da        Isoelectric Point: 5.7066

>AT112037 WP_003382249.1 NZ_CP032631:5361491-5361757 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MTTKTRSVTAHIPEQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSLST
ATPLPVPR

Download         Length: 267 bp

>AT112037 NZ_CP032631:5361491-5361757 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGACAACCAAGACCCGATCTGTAACAGCTCACATACCCGAACAATTGGCTGAAAAAGTCGACCTGATGGCAGAGCGACT
CGAACGCTCTAAAAACTGGATCGTCAAACAGGCCTTGTCTGCATGGATAGATCAGGAGGAAGAACGTAGTCGCCTCACTC
GCGAAGCATTAGCCGATGTCGATGCTGGCCGTGTGATTGACCATCAGGCTGTTCAGGCCTGGGCTGACAGTTTAAGCACC
GCTACACCTCTGCCGGTTCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K8LVV5

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LU58

References