Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 1641466..1642085 Replicon chromosome
Accession NZ_CP032631
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name tad Uniprot ID A0A2G9L4Q9
Locus tag CN228_RS08045 Protein ID WP_004397564.1
Coordinates 1641466..1641768 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0K8LSV9
Locus tag CN228_RS08050 Protein ID WP_004397562.1
Coordinates 1641765..1642085 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS08020 1637241..1637933 - 693 WP_004397574.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
CN228_RS08025 1637947..1638408 - 462 WP_004397572.1 DUF1456 family protein -
CN228_RS08030 1638490..1639200 - 711 WP_004397570.1 rRNA pseudouridine synthase -
CN228_RS08035 1639294..1639734 - 441 WP_017703939.1 GNAT family N-acetyltransferase -
CN228_RS08040 1639836..1641179 - 1344 WP_004397566.1 30S ribosomal protein S12 methylthiotransferase RimO -
CN228_RS08045 1641466..1641768 + 303 WP_004397564.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CN228_RS08050 1641765..1642085 + 321 WP_004397562.1 helix-turn-helix domain-containing protein Antitoxin
CN228_RS08055 1642187..1643275 - 1089 WP_017682838.1 catalase family protein -
CN228_RS08065 1643597..1645492 + 1896 WP_004397556.1 potassium transporter Kup -
CN228_RS08070 1645587..1646921 - 1335 WP_004397555.1 virulence factor family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(1-89)

Antitoxin

HigA2-like, helix-turn-helix domain

(11-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11152.69 Da        Isoelectric Point: 8.0629

>T112035 WP_004397564.1 NZ_CP032631:1641466-1641768 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MPDEVQDVFGFALSLAQEGSKHSQAKPMKGFSGAGVLEVVEDYDSDTYRAVYSVKFDAMVYVLHCFQKKSTSGIKTAKHD
IELIKSRLKAVQAHVESNRR

Download         Length: 303 bp

>T112035 NZ_CP032631:1641466-1641768 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGCCTGACGAGGTGCAGGATGTATTTGGATTCGCCCTGAGTCTCGCGCAGGAAGGCTCCAAACACTCTCAAGCCAAACC
GATGAAGGGTTTCTCCGGAGCAGGGGTGTTGGAGGTCGTTGAGGACTATGATAGTGACACCTATCGCGCTGTATATTCCG
TGAAATTCGATGCAATGGTCTACGTTCTGCACTGTTTTCAGAAAAAGTCGACCTCGGGCATTAAAACGGCAAAACATGAT
ATTGAATTGATCAAATCACGACTCAAAGCCGTACAAGCACATGTAGAGAGTAATAGACGATGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11639.62 Da        Isoelectric Point: 10.2027

>AT112035 WP_004397562.1 NZ_CP032631:1641765-1642085 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MIDIEEGSGNVYADLGRADANEMSIKAQLASKIGTIIKARHLTQLQAAEILAMPQPKVSEMLRGKFRGISEAKMMDCLSR
LGRDVQIVVKAAPRSRREGRVEVVFA

Download         Length: 321 bp

>AT112035 NZ_CP032631:1641765-1642085 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGATTGATATCGAAGAAGGTAGCGGCAACGTTTACGCTGATCTTGGGCGAGCTGATGCGAACGAAATGTCAATCAAGGC
TCAATTAGCCTCAAAAATCGGCACAATCATCAAGGCTCGACACCTGACCCAGCTCCAGGCGGCTGAGATACTGGCAATGC
CTCAGCCAAAGGTTTCAGAGATGCTTAGGGGCAAGTTCAGAGGCATAAGCGAGGCCAAAATGATGGACTGCCTTTCGCGT
CTCGGACGTGACGTGCAGATTGTGGTCAAGGCAGCACCTCGTTCTCGGCGAGAGGGGCGAGTTGAAGTTGTATTTGCTTA
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G9L4Q9

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LSV9

References