Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 738980..739653 Replicon chromosome
Accession NZ_CP032631
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag CN228_RS03705 Protein ID WP_017684243.1
Coordinates 738980..739399 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag CN228_RS03710 Protein ID WP_003381346.1
Coordinates 739396..739653 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS03680 733990..735024 - 1035 WP_017684240.1 sulfotransferase -
CN228_RS03685 735042..735962 - 921 WP_020313563.1 TauD/TfdA family dioxygenase -
CN228_RS03690 736108..736755 - 648 WP_017703697.1 histidine phosphatase family protein -
CN228_RS03695 737096..738031 - 936 WP_017704392.1 syringolide biosynthetic protein AvrD1 -
CN228_RS03700 738306..738939 - 634 Protein_669 recombinase family protein -
CN228_RS03705 738980..739399 - 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
CN228_RS03710 739396..739653 - 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
CN228_RS03715 740016..740534 + 519 WP_017704391.1 hypothetical protein -
CN228_RS03725 742164..742343 + 180 Protein_674 hypothetical protein -
CN228_RS03730 742391..742645 + 255 WP_003381350.1 hypothetical protein -
CN228_RS03740 743340..744302 - 963 WP_003383614.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T112034 WP_017684243.1 NZ_CP032631:c739399-738980 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T112034 NZ_CP032631:c739399-738980 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGATCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT112034 WP_003381346.1 NZ_CP032631:c739653-739396 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT112034 NZ_CP032631:c739653-739396 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References