112033

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 635102..636831 Replicon chromosome
Accession NZ_CP032631
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag CN228_RS03240 Protein ID WP_003379114.1
Coordinates 636187..636831 (-) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag CN228_RS03235 Protein ID WP_003379115.1
Coordinates 635102..636190 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS03210 630433..631209 + 777 WP_017699458.1 ABC transporter permease -
CN228_RS03220 631489..633003 + 1515 WP_017704020.1 IS21-like element ISPsy40 family transposase -
CN228_RS03225 633024..633779 + 756 WP_017683955.1 IS21-like element ISPsy14 family helper ATPase IstB -
CN228_RS03230 633906..634562 + 657 WP_017704180.1 ABC transporter ATP-binding protein -
CN228_RS33530 634643..634896 + 254 Protein_586 glycosyl transferase family 2 -
CN228_RS03235 635102..636190 - 1089 WP_003379115.1 macro domain-containing protein Antitoxin
CN228_RS03240 636187..636831 - 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
CN228_RS03245 636828..637409 - 582 WP_017683790.1 DUF4433 domain-containing protein -
CN228_RS03250 637827..638948 + 1122 WP_003379112.1 DUF91 domain-containing protein -
CN228_RS03255 639248..639979 - 732 WP_003379109.1 helix-turn-helix transcriptional regulator -
CN228_RS03260 640325..640852 - 528 WP_003379107.1 inorganic diphosphatase -
CN228_RS03265 640963..641772 - 810 WP_017683789.1 zinc-dependent peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T112033 WP_003379114.1 NZ_CP032631:c636831-636187 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T112033 NZ_CP032631:c636831-636187 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGATCTACAGCAGTCTGAATCCAGAAAAAGCTCTGATTTGGCGAATCGTCCACCGCGATAATCTGCCTTGGATTCTGGA
CAATGGCCTGCATTGCGCAAGCTCCTACATGCAAGCACCGCAGTACGTGAACATCGGCAACGCTGACCTGATCGACAAAC
GCCGCAATCGCAGTGTTCCAATTGCTCCAGCGGGCGTACTGTCCGACTACGTGCCCTTCTACTTCACACCTTTCTCTGTG
ATGATGAAAAATATCCATTCCGGCTGGAGCGTTCAGCAGCGTAGCAATGACGAGATAGTGATTCTGGTCTCAAGCCTGCC
TCGTATTGAGCAGCTTGGCCTGGCGTTCGTCTTTACCAACGCACACGCCTACCCTGACTGGACCGATTACTACAGCGATC
TGGTGAATCTCGACCAGATCGACTGGCCTATAATCCAGAAACGAGACTTCAAGCGTGACCCTGATGATCCGCGTAAGATG
GAGCGTTATCAGGCCGAAGCGCTCATTCACCATCACCTACCGATTACAGGACTCCTGGGCATCATGTGCTACGACGATGC
AATGAAAGAACGCATTGAGCAAGACGTTGCCGCCCGAGGTCTGACTTTGCCTGTCCACGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT112033 WP_003379115.1 NZ_CP032631:c636190-635102 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp

>AT112033 NZ_CP032631:c636190-635102 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGATCAGATTCACCCAAGGCAATCTGCTGGAAGCAGATACCGAAGCTCTCGTCAACACGGTGAACACTGTAGGCGTAAT
GGGAAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAAAACTATCGCCTCTATTCTGCGGCGTGCAAGGCGGGTG
AGGTAGAGACCGGAAAGGTTCACGTAACGGCAGTCAACGAGCTGGACGGCCCGCGCTGGATAGTGAATTTCCCTACCAAG
CGTCACTGGCGTTCCCCCTCGCAGATGGCATGGATCACCGAAGGCTTACGGGATCTGCGCCGTTTTTTAATCGATAACCA
CGTGAAGTCCGTAGCGGTTCCACCTCTCGGGGCGGGTAATGGCGGTTTGAAATGGGTAGAGGTCCGTGAGCAGATTGTTG
ATGTCTTGAGTGATCTGGATGTCGATGTACTTGTCTTTGAACCCACTGATCAATATCTGAATGTCGCGAAGCGTAGCGGT
GTCGAGAAACTGACGCCTGCACGCGCACTCATTGCCGAACTCGTTCGGCGTTACTGGGTTTTGGGCATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTCGCTTGGTTTCTGGAGCGTTCGATAGAGCAATTGCCAAGCGCCAAGAACCCCTTGGATCTCA
AATTTGTAGCCCACAAATATGGGCCATACGCCAATCGCCTTGAGCATCTGCTCGACAATCTCGATGGTAGCTACCTGCAT
TGCGATAAACGCATCAGCGACGCAGGTATTGACGACGTGATCTGGTTTGACGAGGGCCGTAAGGCTTTTCTGCAAACTTA
TCTGAAAACAGAGGCCAAGGAATATTCCCAGGCTCTGGAGCGCACCGCAGAGTTGATTGACGGCTTTGAGTCGCCTTTCG
GCATGGAACTGTTGGCGACGGTTGACTGGTTATTGTGTAAGTCGGGAGTGTCTCCAACGGTTCCGACAGTGCGTGAAGCA
TTGAAGCATTGGGATGGAGGCGCAGGTGCGGCAGCTCGGAAAAGCAGGTTGTTTGATGATAAAGCGATCGACATTGCTCT
AAAGCGATTGACTACCAGTAGTCTCAGCCCAGAGATGCCCCCTTCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References