Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 252144..252663 Replicon chromosome
Accession NZ_CP032631
Organism Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)


Gene name relE Uniprot ID S6W9N2
Locus tag CN228_RS01160 Protein ID WP_017684310.1
Coordinates 252373..252663 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S6UWW5
Locus tag CN228_RS01155 Protein ID WP_002551444.1
Coordinates 252144..252383 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN228_RS01130 248216..249490 + 1275 WP_003380766.1 OprD family porin -
CN228_RS01135 249576..249923 - 348 WP_017684314.1 helix-turn-helix domain-containing protein -
CN228_RS01140 250196..250690 - 495 WP_005621707.1 hypothetical protein -
CN228_RS01145 250851..251420 + 570 WP_017684312.1 GNAT family N-acetyltransferase -
CN228_RS01150 251507..251968 + 462 WP_017705180.1 GNAT family N-acetyltransferase -
CN228_RS01155 252144..252383 + 240 WP_002551444.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CN228_RS01160 252373..252663 + 291 WP_017684310.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CN228_RS01170 252889..253626 - 738 WP_017684309.1 hypothetical protein -
CN228_RS01175 253983..254324 + 342 WP_003380754.1 hypothetical protein -
CN228_RS01180 254417..255031 + 615 WP_017684308.1 histidine phosphatase family protein -
CN228_RS01185 255212..255454 + 243 WP_017684307.1 hypothetical protein -
CN228_RS01190 255444..255727 + 284 Protein_215 type II toxin-antitoxin system RelE/ParE family toxin -
CN228_RS01195 255791..256333 - 543 WP_017684306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-81)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-64)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11145.98 Da        Isoelectric Point: 10.0984

>T112032 WP_017684310.1 NZ_CP032631:252373-252663 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MTYSLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
RERDEVYRKAADRLGG

Download         Length: 291 bp

>T112032 NZ_CP032631:252373-252663 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGACTTATAGCCTCGAATTTGATGCGCGAGCTCTGAAGGAATGGCACAAATTAGGCGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCAAATCGCCTGCATGCGTTGCCCGACTGCTACAAAATCA
AGCTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGGGTGGGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8955.34 Da        Isoelectric Point: 4.6017

>AT112032 WP_002551444.1 NZ_CP032631:252144-252383 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Download         Length: 240 bp

>AT112032 NZ_CP032631:252144-252383 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGGCGTCTATTAATGTTCGTATCGACGACGATCTCAAGGCTCGTGCTTATCTCGAGCTTGAAAAGCTTGGCGTCACCCC
TTCGGAACTGCTGCGCCAAACCCTGCAGTACGTGGCAGAGCGCGGCCAGTTGCCGTTCAAGACCGTACTGATGACCGAGG
AAGACGAAGCCTTGCTTGCAACAGTGCGCGAGCGCCTTGCGGCTCCGCAACGGGTCAAGGTTTCACTGGATGACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K8M1I3

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8M1G9

References