Detailed information of TA system TA112030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3236685..3237211
• Replicon: chromosome
• Accession: NZ_CP032624
• Organism: Gryllotalpicola protaetiae strain 2DFW10M-5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D7I44_RS15765
• Protein ID: WP_120790367.1
• Coordinates: 3236885..3237211 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D7I44_RS15760
• Protein ID: WP_120790366.1
• Coordinates: 3236685..3236888 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7I44_RS15755	3233149..3236640	+	3492	WP_120790365.1	error-prone DNA polymerase	-
D7I44_RS15760	3236685..3236888	+	204	WP_120790366.1	antitoxin MazE family protein	Antitoxin
D7I44_RS15765	3236885..3237211	+	327	WP_120790367.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D7I44_RS15770	3237262..3237948	+	687	WP_162940326.1	hypothetical protein	-
D7I44_RS15775	3238043..3238546	-	504	WP_120790369.1	sodium:proton antiporter	-
D7I44_RS15780	3238563..3240398	-	1836	WP_120790370.1	DEAD/DEAH box helicase	-
D7I44_RS15785	3240493..3241302	+	810	WP_181445577.1	SGNH/GDSL hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11706.44 Da
• Isoelectric Point: 6.2962

>T112030 WP_120790367.1 NZ_CP032624:3236885-3237211 [Gryllotalpicola protaetiae]
MRRGELWTVSGDVYASKPRPALILQDDRFDATESVTVAPLTTTAVDAPLLRYRLDPAGGNGLDATSFIMVDKLTTVRRNN
IGKQFGSLTGSQLIEVERLVIVFLGLAG

Nucleotide

• Length: 327 bp

>T112030 NZ_CP032624:3236885-3237211 [Gryllotalpicola protaetiae]
ATGAGGCGCGGCGAGCTCTGGACCGTTTCCGGTGACGTCTACGCATCGAAACCGCGCCCGGCGCTGATCCTTCAAGACGA
CCGCTTCGACGCAACCGAGTCGGTCACGGTTGCTCCTCTCACCACAACGGCGGTTGACGCACCCCTTCTCCGTTACCGAC
TTGACCCGGCGGGCGGGAACGGGCTCGACGCGACCAGCTTCATCATGGTCGACAAGCTCACCACGGTGCGCAGGAACAAC
ATCGGCAAGCAGTTCGGCTCGCTCACCGGCAGCCAATTGATCGAGGTGGAGAGATTGGTGATCGTCTTCCTGGGGTTAGC
AGGATGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7770.66 Da
• Isoelectric Point: 5.8213

>AT112030 WP_120790366.1 NZ_CP032624:3236685-3236888 [Gryllotalpicola protaetiae]
MGTRDRVASHRDRMRKAGYRLVQTWVPDVRTPGFVDEARRQAILVAEADRRGEDQDFIEAISAPWDE

Nucleotide

• Length: 204 bp

>AT112030 NZ_CP032624:3236685-3236888 [Gryllotalpicola protaetiae]
ATGGGGACAAGAGACCGCGTGGCAAGTCATCGCGACAGGATGAGGAAGGCGGGGTATCGCCTGGTGCAGACCTGGGTCCC
CGATGTGCGAACACCGGGTTTTGTCGATGAAGCGCGGAGGCAGGCGATCCTTGTCGCCGAGGCCGATCGTCGAGGTGAAG
ACCAGGACTTCATCGAAGCCATTTCTGCGCCGTGGGACGAATGA