Detailed information of TA system TA112029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3203472..3204128
• Replicon: chromosome
• Accession: NZ_CP032624
• Organism: Gryllotalpicola protaetiae strain 2DFW10M-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D7I44_RS15605
• Protein ID: WP_120790339.1
• Coordinates: 3203472..3203870 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D7I44_RS15610
• Protein ID: WP_120790340.1
• Coordinates: 3203874..3204128 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7I44_RS15565	3198754..3199230	-	477	WP_120791003.1	GNAT family N-acetyltransferase	-
D7I44_RS15570	3199289..3199987	-	699	WP_120790333.1	SDR family oxidoreductase	-
D7I44_RS15575	3200030..3200761	+	732	WP_120790334.1	SDR family NAD(P)-dependent oxidoreductase	-
D7I44_RS15580	3200862..3201284	+	423	WP_120791004.1	DUF1801 domain-containing protein	-
D7I44_RS15585	3201508..3201792	+	285	WP_120790335.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D7I44_RS15590	3201789..3202061	+	273	WP_120790336.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D7I44_RS15595	3202227..3202622	+	396	WP_120790337.1	VOC family protein	-
D7I44_RS15600	3202632..3203453	+	822	WP_120790338.1	LLM class F420-dependent oxidoreductase	-
D7I44_RS15605	3203472..3203870	-	399	WP_120790339.1	type II toxin-antitoxin system VapC family toxin	Toxin
D7I44_RS15610	3203874..3204128	-	255	WP_120790340.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
D7I44_RS15615	3204316..3204690	+	375	WP_120790341.1	VOC family protein	-
D7I44_RS15620	3204930..3206348	+	1419	WP_120791005.1	MFS transporter	-
D7I44_RS15625	3206383..3207057	+	675	WP_120790342.1	TetR/AcrR family transcriptional regulator	-
D7I44_RS15630	3207059..3207931	-	873	WP_120790343.1	LLM class flavin-dependent oxidoreductase	-
D7I44_RS15635	3207978..3208646	+	669	WP_120790344.1	4a-hydroxytetrahydrobiopterin dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-127)

Antitoxin

• Antitoxin VapB-like (2-83)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 13713.63 Da
• Isoelectric Point: 4.5580

>T112029 WP_120790339.1 NZ_CP032624:c3203870-3203472 [Gryllotalpicola protaetiae]
MAIAIDTSALIAVIFGEPDASSIARTMLANAGDCSLSAATLVEASIVVEAKQGPAATQDLQLLIERIGAEIMPVDAQTAA
TAISAWRRFGKGRHRAGLNFGDCFAYALSKNSGLPLLFKGDDFTQTDIAQAG

Nucleotide

• Length: 399 bp

>T112029 NZ_CP032624:c3203870-3203472 [Gryllotalpicola protaetiae]
ATGGCGATTGCAATCGATACGTCAGCGCTCATTGCAGTGATTTTCGGCGAACCGGATGCGTCGAGCATCGCGCGCACGAT
GCTCGCGAACGCGGGGGACTGCTCCCTCAGTGCCGCGACCCTCGTTGAGGCTTCGATCGTCGTCGAGGCGAAGCAGGGAC
CGGCAGCCACGCAGGATCTGCAGTTGCTGATCGAGCGGATCGGCGCAGAAATCATGCCGGTCGACGCACAGACCGCCGCG
ACTGCGATTTCGGCCTGGAGACGATTCGGCAAAGGCAGGCACCGCGCCGGGCTCAATTTCGGCGACTGCTTCGCATACGC
ACTGTCCAAGAACTCCGGATTGCCGCTGCTCTTCAAGGGCGACGACTTCACCCAAACAGACATCGCGCAGGCCGGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9033.18 Da
• Isoelectric Point: 5.8507

>AT112029 WP_120790340.1 NZ_CP032624:c3204128-3203874 [Gryllotalpicola protaetiae]
VALNIKDPDTDRLARELAAATGESITVAARVALEERLARVRARTNASLQAADLDAIIERGRARKVIDARSANEILGYDEH
GLPA

Nucleotide

• Length: 255 bp

>AT112029 NZ_CP032624:c3204128-3203874 [Gryllotalpicola protaetiae]
GTGGCGCTCAACATCAAGGATCCGGATACGGACCGCCTCGCGCGCGAACTCGCCGCGGCGACGGGGGAGTCCATCACAGT
GGCCGCTCGGGTCGCGCTTGAAGAAAGGCTGGCGCGAGTTCGCGCACGCACCAATGCGAGTCTTCAAGCTGCCGATCTCG
ACGCGATCATCGAACGCGGGCGGGCACGAAAGGTTATCGACGCCCGGTCTGCGAACGAGATCCTCGGTTACGACGAGCAC
GGACTTCCGGCCTGA