Detailed information of TA system TA112024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 905740..906974
• Replicon: chromosome
• Accession: NZ_CP032621
• Organism: Streptococcus gwangjuense strain KCOM 1679 (=ChDC B345)

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: I0T735
• Locus tag: D7D53_RS04515
• Protein ID: WP_000405378.1
• Coordinates: 905740..906498 (-)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: D7D53_RS04520
• Protein ID: WP_000579616.1
• Coordinates: 906498..906974 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7D53_RS04500	900852..902093	+	1242	WP_120770265.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
D7D53_RS04505	902077..904356	-	2280	WP_050088027.1	ATP-binding protein	-
D7D53_RS04510	904448..905344	+	897	WP_050088029.1	tyrosine-type recombinase/integrase	-
D7D53_RS04515	905740..906498	-	759	WP_000405378.1	zeta toxin family protein	Toxin
D7D53_RS04520	906498..906974	-	477	WP_000579616.1	helix-turn-helix domain-containing protein	Antitoxin
D7D53_RS04525	907262..908623	-	1362	WP_120770266.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
D7D53_RS04530	908697..909671	-	975	WP_001227769.1	DUF1002 domain-containing protein	-
D7D53_RS04535	909745..910359	-	615	WP_120770267.1	cell wall hydrolase Pmp23	-
D7D53_RS04540	910359..911333	-	975	WP_162927871.1	nucleoid-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M) / erm(B)	-	854467..920468	66001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28967.83 Da
• Isoelectric Point: 6.5052

>T112024 WP_000405378.1 NZ_CP032621:c906498-905740 [Streptococcus gwangjuense]
MEIQDYTDSEFKHALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
VYGKDSVEYTKDFAGKMVESLVTELSNLGYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYISTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVHDSREDKISAADILQELLFGEWSQVEKEML
KSGEERFKDLTN

Nucleotide

• Length: 759 bp

>T112024 NZ_CP032621:c906498-905740 [Streptococcus gwangjuense]
ATGGAAATCCAAGATTATACCGATAGTGAATTCAAACATGCTTTAGAGAGGAACCTTCGCTCTCTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCTGGTAAGACTACAATTCATCGAATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GTATATGGTAAAGATAGTGTTGAATACACTAAAGATTTTGCAGGCAAAATGGTGGAGTCTTTAGTAACAGAATTAAGCAA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTAGCCTTAATTGCAACAAAGCCTGAGCTGTCCTATATAAGTACTCTTATCCGTTATGAAGAA
CTGTACGCCATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACCCGACAGTTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTATCAACGAGATAGAAGTTGTGTACATGATTCAA
GAGAAGATAAAATTTCAGCAGCAGATATTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAGGTAGAGAAAGAGATGCTT
AAATCTGGAGAAGAAAGATTTAAAGATTTAACTAATTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18313.77 Da
• Isoelectric Point: 4.4977

>AT112024 WP_000579616.1 NZ_CP032621:c906974-906498 [Streptococcus gwangjuense]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRIYRYQDRQGISIDDESNPWILMSDDLSDLIHTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT112024 NZ_CP032621:c906974-906498 [Streptococcus gwangjuense]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTGAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATAGTGAAAGAAAGAGGTGCTAATCTATTATCTCGAATCTATCGTTATCAAGATAGACAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAGAATTTTGATG
AAATAGAGAGGTATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAA

Structures

Toxin

• AlphaFold DB: I0T735