Detailed information of TA system TA112022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4463447..4464080
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D7Z95_RS20095
• Protein ID: WP_072014142.1
• Coordinates: 4463447..4463623 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D7Z95_RS20100
• Protein ID: WP_264041281.1
• Coordinates: 4463673..4464080 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS20065	4458756..4460925	+	2170	Protein_3908	DNA cytosine methyltransferase	-
D7Z95_RS20070	4460922..4461269	+	348	WP_264041252.1	hypothetical protein	-
D7Z95_RS20075	4461426..4462019	+	594	WP_264041254.1	DUF1367 family protein	-
D7Z95_RS20080	4462016..4462435	+	420	WP_264041256.1	HNH endonuclease	-
D7Z95_RS20085	4462432..4462716	+	285	WP_231051636.1	DUF1364 domain-containing protein	-
D7Z95_RS20090	4462713..4463276	+	564	WP_264041271.1	antiterminator Q family protein	-
D7Z95_RS20095	4463447..4463623	+	177	WP_072014142.1	type II toxin-antitoxin system HicA family toxin	Toxin
D7Z95_RS20100	4463673..4464080	+	408	WP_264041281.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D7Z95_RS20105	4464182..4464397	+	216	WP_137742576.1	class II holin family protein	-
D7Z95_RS20110	4464397..4464939	+	543	WP_264041284.1	lysozyme	-
D7Z95_RS20115	4464936..4465328	+	393	WP_264041286.1	DUF2570 domain-containing protein	-
D7Z95_RS20120	4465462..4465671	-	210	WP_264041289.1	hypothetical protein	-
D7Z95_RS20125	4465671..4465874	-	204	WP_264041291.1	YccJ family protein	-
D7Z95_RS20130	4466238..4466492	+	255	WP_264041293.1	DNA polymerase III subunit theta	-
D7Z95_RS20135	4466703..4467335	+	633	WP_264041294.1	putative metallopeptidase	-
D7Z95_RS20140	4467367..4467852	+	486	WP_264041296.1	DUF2280 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4444682..4490204	45522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-63)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.79 Da
• Isoelectric Point: 11.3427

>T112022 WP_072014142.1 NZ_CP032619:4463447-4463623 [Pectobacterium carotovorum]
VKQSEFRRWLESQGVEVSNGTNHLKLRYNGNRSVMPRHPSAEIKEPLRKAILKQLGLK

Nucleotide

• Length: 177 bp

>T112022 NZ_CP032619:4463447-4463623 [Pectobacterium carotovorum]
GTGAAGCAAAGCGAGTTCAGGCGGTGGCTTGAATCTCAGGGGGTTGAGGTTTCAAACGGTACAAATCATTTGAAGCTGAG
ATACAACGGGAACCGAAGCGTGATGCCGAGGCACCCGAGCGCTGAGATAAAAGAACCACTGCGAAAGGCCATACTGAAAC
AGCTTGGCCTGAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14756.80 Da
• Isoelectric Point: 4.3759

>AT112022 WP_264041281.1 NZ_CP032619:4463673-4464080 [Pectobacterium carotovorum]
MRYPVNLDPDSGGYVVSFPDIPEALTQGDTREDALKEALDALVTAFEFYFEDNQRVPAPGNITGDYVEVPASIAAKVLML
NAFVDSGLTQVELASRMGVKKQEVTRLFDLHHSTKIDTIQKAISAMGLRLELVAA

Nucleotide

• Length: 408 bp

>AT112022 NZ_CP032619:4463673-4464080 [Pectobacterium carotovorum]
ATGCGATATCCAGTAAATTTAGACCCGGATTCAGGCGGCTATGTTGTATCGTTTCCTGATATACCGGAAGCCCTGACGCA
AGGGGATACGCGGGAAGACGCGTTAAAAGAGGCGCTGGATGCGCTGGTAACAGCCTTCGAGTTTTATTTCGAGGATAACC
AGCGCGTTCCGGCACCCGGTAATATAACGGGTGATTACGTCGAAGTTCCGGCAAGTATCGCCGCTAAAGTTTTAATGCTT
AATGCGTTTGTCGATTCCGGCTTAACTCAGGTTGAGCTGGCTTCGCGCATGGGTGTAAAGAAGCAGGAGGTAACCCGCCT
GTTTGACCTACACCACTCGACGAAAATAGACACCATTCAAAAGGCGATCTCGGCTATGGGATTGCGTCTGGAATTGGTCG
CCGCGTAA