Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3749978..3750603 Replicon chromosome
Accession NZ_CP032619
Organism Pectobacterium carotovorum strain HG-49

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag D7Z95_RS16830 Protein ID WP_005976087.1
Coordinates 3749978..3750181 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C6DB73
Locus tag D7Z95_RS16835 Protein ID WP_005976089.1
Coordinates 3750235..3750603 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7Z95_RS16800 3745420..3745758 + 339 WP_002208627.1 P-II family nitrogen regulator -
D7Z95_RS16805 3745796..3747082 + 1287 WP_015839357.1 ammonium transporter AmtB -
D7Z95_RS16810 3747235..3748098 - 864 WP_015839358.1 acyl-CoA thioesterase II -
D7Z95_RS16815 3748310..3748891 + 582 WP_015839359.1 YbaY family lipoprotein -
D7Z95_RS16820 3748911..3749231 - 321 WP_015839360.1 MGMT family protein -
D7Z95_RS16830 3749978..3750181 - 204 WP_005976087.1 HHA domain-containing protein Toxin
D7Z95_RS16835 3750235..3750603 - 369 WP_005976089.1 Hha toxicity modulator TomB Antitoxin
D7Z95_RS16840 3751200..3751343 - 144 WP_005976091.1 type B 50S ribosomal protein L36 -
D7Z95_RS16845 3751361..3751609 - 249 WP_015839361.1 type B 50S ribosomal protein L31 -
D7Z95_RS16850 3751844..3754972 - 3129 WP_015839362.1 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T112020 WP_005976087.1 NZ_CP032619:c3750181-3749978 [Pectobacterium carotovorum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T112020 NZ_CP032619:c3750181-3749978 [Pectobacterium carotovorum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TTTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14032.77 Da        Isoelectric Point: 4.4787

>AT112020 WP_005976089.1 NZ_CP032619:c3750603-3750235 [Pectobacterium carotovorum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Download         Length: 369 bp

>AT112020 NZ_CP032619:c3750603-3750235 [Pectobacterium carotovorum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGGATTAATGATGCTGAACTGCGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7Z3G5

References