Detailed information of TA system TA112019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3514671..3515085
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: D7Z95_RS15815
• Protein ID: WP_241007237.1
• Coordinates: 3514671..3514784 (-)
• Length: 38 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: D7Z95_RS15820
• Protein ID: WP_205947011.1
• Coordinates: 3514867..3515085 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS15800	3509811..3510305	+	495	WP_205947013.1	GNAT family N-acetyltransferase	-
D7Z95_RS15805	3510577..3513090	+	2514	WP_205947012.1	ATP-binding protein	-
D7Z95_RS15810	3513548..3514659	+	1112	WP_205946711.1	IS3 family transposase	-
D7Z95_RS15815	3514671..3514784	-	114	WP_241007237.1	hypothetical protein	Toxin
D7Z95_RS15820	3514867..3515085	-	219	WP_205947011.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
D7Z95_RS15825	3515100..3515348	-	249	WP_205947010.1	helix-turn-helix transcriptional regulator	-
D7Z95_RS15830	3515434..3515688	-	255	WP_205947009.1	helix-turn-helix transcriptional regulator	-
D7Z95_RS15835	3515792..3516034	+	243	WP_205947008.1	helix-turn-helix transcriptional regulator	-
D7Z95_RS15845	3516640..3516771	+	132	WP_256553759.1	hypothetical protein	-
D7Z95_RS15850	3517003..3519156	+	2154	WP_205947007.1	ornithine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3495161..3516034	20873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 38 a.a.
• Molecular weight: 4382.16 Da
• Isoelectric Point: 6.4501

>T112019 WP_241007237.1 NZ_CP032619:c3514784-3514671 [Pectobacterium carotovorum]
MVIPLIESHHLSEKVNKTLFRLIRIDGEDYRLMATEL

Nucleotide

• Length: 114 bp

>T112019 NZ_CP032619:c3514784-3514671 [Pectobacterium carotovorum]
ATGGTCATTCCACTTATCGAATCGCATCATCTGTCTGAGAAAGTGAATAAGACGTTGTTCCGTCTGATCCGCATCGATGG
CGAAGATTATCGGCTGATGGCCACGGAGCTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8211.24 Da
• Isoelectric Point: 8.5958

>AT112019 WP_205947011.1 NZ_CP032619:c3515085-3514867 [Pectobacterium carotovorum]
MKRRISIAVDKDDYQILSAAGVNISELVNDALGKEARRIKTEAWKKENREGMENVAQFIAKNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT112019 NZ_CP032619:c3515085-3514867 [Pectobacterium carotovorum]
ATGAAACGCCGGATTAGCATTGCCGTGGACAAAGATGACTATCAGATTCTGAGTGCTGCCGGAGTTAATATTTCCGAGCT
GGTGAATGACGCCCTTGGCAAAGAAGCCCGCCGCATAAAGACTGAGGCGTGGAAGAAGGAAAACCGTGAAGGGATGGAAA
ACGTCGCCCAATTTATCGCAAAGAATGGTTCTTTTGCGGATGAAAACAGGAACTGGTGA